Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22568 | 3' | -55.4 | NC_005091.1 | + | 15397 | 0.66 | 0.710854 |
Target: 5'- -gUCGCU-CGUGG-UGGUAACGUCc- -3' miRNA: 3'- uuGGCGAaGCACCuGCCGUUGCAGcu -5' |
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22568 | 3' | -55.4 | NC_005091.1 | + | 28052 | 0.66 | 0.700121 |
Target: 5'- ---gGCUUCGUGu-CGGUGAUGUCGGc -3' miRNA: 3'- uuggCGAAGCACcuGCCGUUGCAGCU- -5' |
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22568 | 3' | -55.4 | NC_005091.1 | + | 33039 | 0.66 | 0.700121 |
Target: 5'- cGCCGCUcUCGcGGAUGaUGAUGUCGAa -3' miRNA: 3'- uUGGCGA-AGCaCCUGCcGUUGCAGCU- -5' |
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22568 | 3' | -55.4 | NC_005091.1 | + | 55354 | 0.66 | 0.699044 |
Target: 5'- aGACCGaaUCGaagacgugccgacUGGAUGGCAACGaggCGAc -3' miRNA: 3'- -UUGGCgaAGC-------------ACCUGCCGUUGCa--GCU- -5' |
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22568 | 3' | -55.4 | NC_005091.1 | + | 11422 | 0.67 | 0.667571 |
Target: 5'- -cCUGUUUCGcGG-CGGCcACGUCGGc -3' miRNA: 3'- uuGGCGAAGCaCCuGCCGuUGCAGCU- -5' |
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22568 | 3' | -55.4 | NC_005091.1 | + | 25100 | 0.67 | 0.667571 |
Target: 5'- uGACCGCUgCGUucacgGGAUGGCAGa-UCGGg -3' miRNA: 3'- -UUGGCGAaGCA-----CCUGCCGUUgcAGCU- -5' |
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22568 | 3' | -55.4 | NC_005091.1 | + | 50768 | 0.67 | 0.645688 |
Target: 5'- gAGCCGCagUUCGcGGGCcagcuuGGCAACGcUCGGc -3' miRNA: 3'- -UUGGCG--AAGCaCCUG------CCGUUGC-AGCU- -5' |
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22568 | 3' | -55.4 | NC_005091.1 | + | 53902 | 0.67 | 0.634723 |
Target: 5'- aAACCGCUUCGUcGGACaGCGgauuGCGcCa- -3' miRNA: 3'- -UUGGCGAAGCA-CCUGcCGU----UGCaGcu -5' |
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22568 | 3' | -55.4 | NC_005091.1 | + | 20002 | 0.68 | 0.612796 |
Target: 5'- cACUGC--CGUGGGCGGCGAUugguUCGAg -3' miRNA: 3'- uUGGCGaaGCACCUGCCGUUGc---AGCU- -5' |
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22568 | 3' | -55.4 | NC_005091.1 | + | 48621 | 0.69 | 0.558423 |
Target: 5'- gGAUCGC-UCG-GGGCGGC--CGUCGGg -3' miRNA: 3'- -UUGGCGaAGCaCCUGCCGuuGCAGCU- -5' |
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22568 | 3' | -55.4 | NC_005091.1 | + | 45933 | 0.7 | 0.49516 |
Target: 5'- cGGCCGCUUCGUGucCGGaugGAUGUgGAa -3' miRNA: 3'- -UUGGCGAAGCACcuGCCg--UUGCAgCU- -5' |
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22568 | 3' | -55.4 | NC_005091.1 | + | 19143 | 0.7 | 0.455825 |
Target: 5'- cACCGUgggcgaucuuacugCGUGGGCgGGCAGCGUCu- -3' miRNA: 3'- uUGGCGaa------------GCACCUG-CCGUUGCAGcu -5' |
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22568 | 3' | -55.4 | NC_005091.1 | + | 17989 | 0.71 | 0.41636 |
Target: 5'- -uCCGCUucaacguacgUCGUGGACGGCAGac-CGAa -3' miRNA: 3'- uuGGCGA----------AGCACCUGCCGUUgcaGCU- -5' |
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22568 | 3' | -55.4 | NC_005091.1 | + | 49819 | 0.76 | 0.202929 |
Target: 5'- cGCCGCgUUCGUGG-CGGCuugcGCGUUGAg -3' miRNA: 3'- uUGGCG-AAGCACCuGCCGu---UGCAGCU- -5' |
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22568 | 3' | -55.4 | NC_005091.1 | + | 14172 | 1.07 | 0.001559 |
Target: 5'- gAACCGCUUCGUGGACGGCAACGUCGAu -3' miRNA: 3'- -UUGGCGAAGCACCUGCCGUUGCAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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