Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 22569 | 3' | -55.5 | NC_005091.1 | + | 56451 | 0.66 | 0.754528 |
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Target: 5'- -cUGCGGCugGCGuauggggcuucuGGCGGgaCGGCa -3' miRNA: 3'- gcAUGCUGugCGUu-----------CCGCCgaGCUGg -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 44134 | 0.66 | 0.746382 |
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Target: 5'- aGUACGuCGCGCAgucugucgaauGGGCGcagucGCUucccggCGACCu -3' miRNA: 3'- gCAUGCuGUGCGU-----------UCCGC-----CGA------GCUGG- -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 21639 | 0.66 | 0.745359 |
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Target: 5'- aCGUACGACAucaugguCGCca-GCGGCUacaccccgGACCa -3' miRNA: 3'- -GCAUGCUGU-------GCGuucCGCCGAg-------CUGG- -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 5098 | 0.66 | 0.725717 |
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Target: 5'- ---uUGACACGCGuuGCGGCgcaggacgCGAUCg -3' miRNA: 3'- gcauGCUGUGCGUucCGCCGa-------GCUGG- -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 9240 | 0.66 | 0.756553 |
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Target: 5'- gGUGCGGCccuGCGaAGGGCGaC-CGGCCg -3' miRNA: 3'- gCAUGCUG---UGCgUUCCGCcGaGCUGG- -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 55582 | 0.66 | 0.724673 |
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Target: 5'- --cGCGGucCACGCGcucgugcAGGCGGCgaUCGGCg -3' miRNA: 3'- gcaUGCU--GUGCGU-------UCCGCCG--AGCUGg -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 30086 | 0.66 | 0.725717 |
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Target: 5'- --gACGGaccaGCGCuuuaAGGGCGGCgaagucgcCGACCa -3' miRNA: 3'- gcaUGCUg---UGCG----UUCCGCCGa-------GCUGG- -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 49769 | 0.66 | 0.725717 |
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Target: 5'- aCGgcuUGGCAUGCAGcacgcuCGGCUCGAUCg -3' miRNA: 3'- -GCau-GCUGUGCGUUcc----GCCGAGCUGG- -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 34099 | 0.66 | 0.7361 |
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Target: 5'- gCGUACGACGCGUugauGaCGGCguuuugaagCGACa -3' miRNA: 3'- -GCAUGCUGUGCGuu--CcGCCGa--------GCUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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