Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22569 | 3' | -55.5 | NC_005091.1 | + | 52670 | 0.72 | 0.387588 |
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Target: 5'- gGUACGACACGaccagacGGGCGcGCacgcCGGCCa -3' miRNA: 3'- gCAUGCUGUGCgu-----UCCGC-CGa---GCUGG- -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 52733 | 0.67 | 0.708921 |
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Target: 5'- uCGUACcucauccucgguucgGGCAuCGCuuGGGCGGCugUCGGCUu -3' miRNA: 3'- -GCAUG---------------CUGU-GCGu-UCCGCCG--AGCUGG- -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 54409 | 0.72 | 0.423934 |
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Target: 5'- aCGgACGACauGCGCGAaGC-GCUCGACCa -3' miRNA: 3'- -GCaUGCUG--UGCGUUcCGcCGAGCUGG- -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 54517 | 0.76 | 0.250894 |
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Target: 5'- aCGUcCGACACGgAAGGCGGCgaaACCc -3' miRNA: 3'- -GCAuGCUGUGCgUUCCGCCGagcUGG- -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 55210 | 0.68 | 0.629495 |
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Target: 5'- --cGCGGCccguGCGCGGGGgucCGGCUCGGuucCCg -3' miRNA: 3'- gcaUGCUG----UGCGUUCC---GCCGAGCU---GG- -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 55397 | 0.7 | 0.521852 |
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Target: 5'- --cGCGAgCACGC-GGGCGGCaaagcugUCGAUCu -3' miRNA: 3'- gcaUGCU-GUGCGuUCCGCCG-------AGCUGG- -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 55582 | 0.66 | 0.724673 |
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Target: 5'- --cGCGGucCACGCGcucgugcAGGCGGCgaUCGGCg -3' miRNA: 3'- gcaUGCU--GUGCGU-------UCCGCCG--AGCUGg -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 56451 | 0.66 | 0.754528 |
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Target: 5'- -cUGCGGCugGCGuauggggcuucuGGCGGgaCGGCa -3' miRNA: 3'- gcAUGCUGugCGUu-----------CCGCCgaGCUGg -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 57768 | 0.67 | 0.715244 |
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Target: 5'- cCGU-CGACGcCGUcgugAAGGCGGCcaagCGGCg -3' miRNA: 3'- -GCAuGCUGU-GCG----UUCCGCCGa---GCUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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