Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22569 | 3' | -55.5 | NC_005091.1 | + | 22034 | 0.67 | 0.704691 |
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Target: 5'- uGUAuCGuCGCGUAuuggGGGUaacGCUCGACCa -3' miRNA: 3'- gCAU-GCuGUGCGU----UCCGc--CGAGCUGG- -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 14086 | 0.67 | 0.682321 |
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Target: 5'- -aUAUGugGCGCGcgaucuccggaacGGGCGGUgCGACg -3' miRNA: 3'- gcAUGCugUGCGU-------------UCCGCCGaGCUGg -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 24627 | 0.67 | 0.661904 |
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Target: 5'- ---cCGAUACGUccGGCGGCUUGuACg -3' miRNA: 3'- gcauGCUGUGCGuuCCGCCGAGC-UGg -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 51550 | 0.67 | 0.661904 |
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Target: 5'- cCGcGCGGCAuCGCcaaguGGGCGGaagcgaCGACCg -3' miRNA: 3'- -GCaUGCUGU-GCGu----UCCGCCga----GCUGG- -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 9057 | 0.68 | 0.651114 |
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Target: 5'- uGUACGACGCGCucgccGGGaaGUaaCGGCCg -3' miRNA: 3'- gCAUGCUGUGCGu----UCCgcCGa-GCUGG- -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 13067 | 0.68 | 0.651114 |
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Target: 5'- --gGCGGCAgGCAAuacGCcGCUCGGCCc -3' miRNA: 3'- gcaUGCUGUgCGUUc--CGcCGAGCUGG- -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 22142 | 0.68 | 0.640308 |
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Target: 5'- -aUACGACgaucaccaucaaGCGCA--GCGGCUUGGCUg -3' miRNA: 3'- gcAUGCUG------------UGCGUucCGCCGAGCUGG- -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 16564 | 0.68 | 0.640308 |
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Target: 5'- aGUACG-CGCGgAAGaauucCGGCUgGGCCg -3' miRNA: 3'- gCAUGCuGUGCgUUCc----GCCGAgCUGG- -5' |
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| 22569 | 3' | -55.5 | NC_005091.1 | + | 8112 | 0.66 | 0.7666 |
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Target: 5'- uCGaACGACAgGUAAuGCaGGC-CGACCu -3' miRNA: 3'- -GCaUGCUGUgCGUUcCG-CCGaGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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