Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22569 | 5' | -54.2 | NC_005091.1 | + | 25488 | 0.66 | 0.774938 |
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Target: 5'- gGCGAUcGcCGaUAUgGGCUUCGCGGa--- -3' miRNA: 3'- -UGCUA-C-GC-AUAgCCGAAGCGCCacag -5' |
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| 22569 | 5' | -54.2 | NC_005091.1 | + | 32570 | 0.66 | 0.754482 |
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Target: 5'- gACGAUGCGcUGaaccUCgGGCUgauugaCGCGGUGg- -3' miRNA: 3'- -UGCUACGC-AU----AG-CCGAa-----GCGCCACag -5' |
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| 22569 | 5' | -54.2 | NC_005091.1 | + | 34932 | 0.67 | 0.711077 |
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Target: 5'- -aGgcGCGUcgCGGCUcUCGCGagagaccGUGUCg -3' miRNA: 3'- ugCuaCGCAuaGCCGA-AGCGC-------CACAG- -5' |
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| 22569 | 5' | -54.2 | NC_005091.1 | + | 4916 | 0.68 | 0.668554 |
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Target: 5'- cUGAUGCGauUCGGCUaCGCGGa--- -3' miRNA: 3'- uGCUACGCauAGCCGAaGCGCCacag -5' |
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| 22569 | 5' | -54.2 | NC_005091.1 | + | 24555 | 0.69 | 0.624398 |
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Target: 5'- cCGccgGaCGUAUCGGUca-GCGGUGUCg -3' miRNA: 3'- uGCua-C-GCAUAGCCGaagCGCCACAG- -5' |
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| 22569 | 5' | -54.2 | NC_005091.1 | + | 44028 | 0.71 | 0.494788 |
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Target: 5'- cACGAUGgaCGUGggCGGCUUCGaaccCGGUGUg -3' miRNA: 3'- -UGCUAC--GCAUa-GCCGAAGC----GCCACAg -5' |
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| 22569 | 5' | -54.2 | NC_005091.1 | + | 14288 | 1.1 | 0.001168 |
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Target: 5'- cACGAUGCGUAUCGGCUUCGCGGUGUCg -3' miRNA: 3'- -UGCUACGCAUAGCCGAAGCGCCACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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