Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2257 | 3' | -51.3 | NC_001405.1 | + | 17833 | 0.66 | 0.814316 |
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Target: 5'- cGACGgugCGacgcGCgCCGCCGCCGGUGGu -3' miRNA: 3'- -CUGCaa-GUcua-UG-GGUGGUGGUCAUC- -5' |
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| 2257 | 3' | -51.3 | NC_001405.1 | + | 8151 | 0.67 | 0.728944 |
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Target: 5'- gGAUGUaCAGcccGUGCaCCGCUGCCAGUAc -3' miRNA: 3'- -CUGCAaGUC---UAUG-GGUGGUGGUCAUc -5' |
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| 2257 | 3' | -51.3 | NC_001405.1 | + | 12239 | 0.68 | 0.671587 |
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Target: 5'- cGCGcgCGGGUG-CCACCGCCAGc-- -3' miRNA: 3'- cUGCaaGUCUAUgGGUGGUGGUCauc -5' |
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| 2257 | 3' | -51.3 | NC_001405.1 | + | 22092 | 0.69 | 0.648257 |
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Target: 5'- aGCGUgCAGggGCCCaacucgGCCGCCuGUGGa -3' miRNA: 3'- cUGCAaGUCuaUGGG------UGGUGGuCAUC- -5' |
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| 2257 | 3' | -51.3 | NC_001405.1 | + | 26644 | 0.7 | 0.589845 |
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Target: 5'- uGGCGUUCuuGUGCCCgcGCCACgagCGGUAGc -3' miRNA: 3'- -CUGCAAGucUAUGGG--UGGUG---GUCAUC- -5' |
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| 2257 | 3' | -51.3 | NC_001405.1 | + | 25599 | 0.77 | 0.227229 |
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Target: 5'- aGCGUUCAGGaauucUGCCCGCCACCugcuGUGc -3' miRNA: 3'- cUGCAAGUCU-----AUGGGUGGUGGu---CAUc -5' |
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| 2257 | 3' | -51.3 | NC_001405.1 | + | 17172 | 1.08 | 0.001691 |
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Target: 5'- gGACGUUCAGAUACCCACCACCAGUAGc -3' miRNA: 3'- -CUGCAAGUCUAUGGGUGGUGGUCAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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