Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2257 | 5' | -58.1 | NC_001405.1 | + | 23569 | 0.66 | 0.399363 |
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Target: 5'- cUGCCGaucguguCUGCgugGgAGaAGGGCAUGGAGGc -3' miRNA: 3'- -ACGGU-------GACGg--UgUC-UCCCGUACCUCU- -5' |
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| 2257 | 5' | -58.1 | NC_001405.1 | + | 31542 | 0.66 | 0.381722 |
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Target: 5'- -aCUACUGCCACGGGuagcuuGGGCAuuaacaUGGaAGAu -3' miRNA: 3'- acGGUGACGGUGUCU------CCCGU------ACC-UCU- -5' |
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| 2257 | 5' | -58.1 | NC_001405.1 | + | 9064 | 0.67 | 0.354946 |
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Target: 5'- cGCCcgcgaUGCCGaAGGGGGCGUGGu-- -3' miRNA: 3'- aCGGug---ACGGUgUCUCCCGUACCucu -5' |
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| 2257 | 5' | -58.1 | NC_001405.1 | + | 15145 | 0.67 | 0.3246 |
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Target: 5'- aUGCCAUucgcggcgacaccuuUGCCACAc-GGGCGgaGGAGAa -3' miRNA: 3'- -ACGGUG---------------ACGGUGUcuCCCGUa-CCUCU- -5' |
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| 2257 | 5' | -58.1 | NC_001405.1 | + | 23908 | 0.69 | 0.261448 |
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Target: 5'- cGCCGCcucaGCCGCuuuuuuGGGGGCGcgcGGGGAg -3' miRNA: 3'- aCGGUGa---CGGUGu-----CUCCCGUa--CCUCU- -5' |
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| 2257 | 5' | -58.1 | NC_001405.1 | + | 31542 | 0.69 | 0.247968 |
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Target: 5'- cUGUCACUGCCAgAGAGGGg--GGcuGAu -3' miRNA: 3'- -ACGGUGACGGUgUCUCCCguaCCu-CU- -5' |
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| 2257 | 5' | -58.1 | NC_001405.1 | + | 26789 | 0.7 | 0.216778 |
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Target: 5'- cUGCCGCUGCCgccgguGCAGuaGGGGCuguaGAGAu -3' miRNA: 3'- -ACGGUGACGG------UGUC--UCCCGuac-CUCU- -5' |
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| 2257 | 5' | -58.1 | NC_001405.1 | + | 5510 | 0.73 | 0.12731 |
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Target: 5'- gGCCAC-GCCGCGGAGGGgcUGGAc- -3' miRNA: 3'- aCGGUGaCGGUGUCUCCCguACCUcu -5' |
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| 2257 | 5' | -58.1 | NC_001405.1 | + | 17207 | 1.08 | 0.000264 |
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Target: 5'- uUGCCACUGCCACAGAGGGCAUGGAGAc -3' miRNA: 3'- -ACGGUGACGGUGUCUCCCGUACCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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