Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22570 | 3' | -55.6 | NC_005091.1 | + | 20224 | 0.68 | 0.646529 |
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Target: 5'- aCGAuGUCCAguGAagCCGcGCaGUCGAGCg -3' miRNA: 3'- gGCUcCAGGU--UUagGGC-CG-CAGCUCG- -5' |
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| 22570 | 3' | -55.6 | NC_005091.1 | + | 51789 | 0.68 | 0.646529 |
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Target: 5'- uCCGcGaUCU---UCCCGGCGUcCGGGCg -3' miRNA: 3'- -GGCuCcAGGuuuAGGGCCGCA-GCUCG- -5' |
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| 22570 | 3' | -55.6 | NC_005091.1 | + | 701 | 0.68 | 0.635787 |
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Target: 5'- uCCGcaguGGUCgAuGUCCgGGCugcugcgugagGUCGAGCg -3' miRNA: 3'- -GGCu---CCAGgUuUAGGgCCG-----------CAGCUCG- -5' |
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| 22570 | 3' | -55.6 | NC_005091.1 | + | 31761 | 0.71 | 0.44954 |
|
Target: 5'- uCCGAGGcggCCGGGuUCCaCGGCa-CGAGCa -3' miRNA: 3'- -GGCUCCa--GGUUU-AGG-GCCGcaGCUCG- -5' |
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| 22570 | 3' | -55.6 | NC_005091.1 | + | 11370 | 0.71 | 0.44954 |
|
Target: 5'- uCCGcuGUuuGAGUCCgGGCGUCGGccGCg -3' miRNA: 3'- -GGCucCAggUUUAGGgCCGCAGCU--CG- -5' |
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| 22570 | 3' | -55.6 | NC_005091.1 | + | 32493 | 0.72 | 0.410258 |
|
Target: 5'- cCCGAGGUUCAGcgcaucgucugCCCGGUacGUCuGAGCc -3' miRNA: 3'- -GGCUCCAGGUUua---------GGGCCG--CAG-CUCG- -5' |
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| 22570 | 3' | -55.6 | NC_005091.1 | + | 18151 | 0.73 | 0.368075 |
|
Target: 5'- gCGAGcUUCGcggCCCGGCGaUCGAGCg -3' miRNA: 3'- gGCUCcAGGUuuaGGGCCGC-AGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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