miRNA: 3'- gACAGCCUGCG-GUUGUagaGCu---UGGG- -5'
miRNA: 3'- gACaGCC--UGCgGUU-GUAGAGCUugGG- -5'
miRNA: 3'- gaCAGCCU------------GCGGUugUAG----------AGCuUGGG- -5'
miRNA: 3'- gaCAGCCUGCGgU--------UGUAG----------AGCU--UGGG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22570 | 5' | -52.7 | NC_005091.1 | + | 10427 | 0.66 | 0.891936 |
|
Target: 5'- aUGUCGGGCGCgCGACAcg-CGcaguACUCu -3' miRNA: 3'- gACAGCCUGCG-GUUGUagaGCu---UGGG- -5' |
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| 22570 | 5' | -52.7 | NC_005091.1 | + | 7174 | 0.66 | 0.852665 |
|
Target: 5'- uUGcCGGcaGCGgCGGcCGUCUCGAugCCc -3' miRNA: 3'- gACaGCC--UGCgGUU-GUAGAGCUugGG- -5' |
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| 22570 | 5' | -52.7 | NC_005091.1 | + | 7002 | 0.67 | 0.817033 |
|
Target: 5'- -gGUCGGAgauguucaguucCGCCAugAUCucuuucuucgUCGcGCCCg -3' miRNA: 3'- gaCAGCCU------------GCGGUugUAG----------AGCuUGGG- -5' |
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| 22570 | 5' | -52.7 | NC_005091.1 | + | 6146 | 0.7 | 0.694216 |
|
Target: 5'- -aGUCGGACGCgAacuuucugGCGUCuccgucguccUCGAacGCCCg -3' miRNA: 3'- gaCAGCCUGCGgU--------UGUAG----------AGCU--UGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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