Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22573 | 3' | -48 | NC_005091.1 | + | 19514 | 0.66 | 0.989887 |
Target: 5'- gUGCAGCGAUuggacgGUCGUA--GGUCU-CGg -3' miRNA: 3'- aGCGUUGCUA------UAGCAUacCCAGAuGC- -5' |
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22573 | 3' | -48 | NC_005091.1 | + | 6655 | 0.66 | 0.988359 |
Target: 5'- uUCGCGAUGucuUCGaUGUGGGcCUuCGa -3' miRNA: 3'- -AGCGUUGCuauAGC-AUACCCaGAuGC- -5' |
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22573 | 3' | -48 | NC_005091.1 | + | 12720 | 0.67 | 0.977895 |
Target: 5'- gCGUGugGAUcgacaAUCGgcUGGGUC-GCGa -3' miRNA: 3'- aGCGUugCUA-----UAGCauACCCAGaUGC- -5' |
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22573 | 3' | -48 | NC_005091.1 | + | 20129 | 0.67 | 0.976281 |
Target: 5'- -gGCAGCaGAUGUCG-AUGacguucaagcgcggcGGUCUGCGc -3' miRNA: 3'- agCGUUG-CUAUAGCaUAC---------------CCAGAUGC- -5' |
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22573 | 3' | -48 | NC_005091.1 | + | 36459 | 0.67 | 0.972182 |
Target: 5'- gCGCAACGAUcUCGaccUGUGGGgcgUCUGg- -3' miRNA: 3'- aGCGUUGCUAuAGC---AUACCC---AGAUgc -5' |
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22573 | 3' | -48 | NC_005091.1 | + | 8990 | 0.68 | 0.961727 |
Target: 5'- cCGCAGCu---UCGUAcGGGUCUuCGg -3' miRNA: 3'- aGCGUUGcuauAGCAUaCCCAGAuGC- -5' |
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22573 | 3' | -48 | NC_005091.1 | + | 2394 | 0.68 | 0.953404 |
Target: 5'- cUCGCAACGGg--CGUA---GUCUACGa -3' miRNA: 3'- -AGCGUUGCUauaGCAUaccCAGAUGC- -5' |
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22573 | 3' | -48 | NC_005091.1 | + | 7884 | 0.73 | 0.797259 |
Target: 5'- cUCGCGACGGU-UCGauggGUGGGUCcAUGu -3' miRNA: 3'- -AGCGUUGCUAuAGCa---UACCCAGaUGC- -5' |
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22573 | 3' | -48 | NC_005091.1 | + | 47387 | 0.75 | 0.655325 |
Target: 5'- aUGCGGCGGUguuuAUCGUGUuguacGGGUCUACc -3' miRNA: 3'- aGCGUUGCUA----UAGCAUA-----CCCAGAUGc -5' |
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22573 | 3' | -48 | NC_005091.1 | + | 2811 | 0.76 | 0.598186 |
Target: 5'- uUCGCGAgaGAUAUCGUccGGGUCaucgGCGa -3' miRNA: 3'- -AGCGUUg-CUAUAGCAuaCCCAGa---UGC- -5' |
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22573 | 3' | -48 | NC_005091.1 | + | 17603 | 1.11 | 0.004896 |
Target: 5'- uUCGCAACGAUAUCGUAUGGGUCUACGa -3' miRNA: 3'- -AGCGUUGCUAUAGCAUACCCAGAUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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