Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22573 | 5' | -57.4 | NC_005091.1 | + | 17865 | 0.66 | 0.662852 |
Target: 5'- cGGCcgGGACCUGUgCGAGaUCGGCc- -3' miRNA: 3'- -CCGcgUCUGGGCAgGCUCaAGUCGcu -5' |
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22573 | 5' | -57.4 | NC_005091.1 | + | 34563 | 0.66 | 0.662852 |
Target: 5'- cGGCGaAGugCUGgcgacugCCGAGUgggaccgcgCGGCGAa -3' miRNA: 3'- -CCGCgUCugGGCa------GGCUCAa--------GUCGCU- -5' |
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22573 | 5' | -57.4 | NC_005091.1 | + | 741 | 0.66 | 0.652155 |
Target: 5'- cGCGUAGauuuccgcgauGCCCGgucgauucUCCGcGUUCAGCGc -3' miRNA: 3'- cCGCGUC-----------UGGGC--------AGGCuCAAGUCGCu -5' |
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22573 | 5' | -57.4 | NC_005091.1 | + | 3380 | 0.66 | 0.652155 |
Target: 5'- cGCGCAGcuuCUCG-CCGAGUUCuucGCa- -3' miRNA: 3'- cCGCGUCu--GGGCaGGCUCAAGu--CGcu -5' |
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22573 | 5' | -57.4 | NC_005091.1 | + | 29707 | 0.66 | 0.648942 |
Target: 5'- cGCGCAGcguacggcccucccGCUCGcccgcaCCGAGaUCGGCGAg -3' miRNA: 3'- cCGCGUC--------------UGGGCa-----GGCUCaAGUCGCU- -5' |
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22573 | 5' | -57.4 | NC_005091.1 | + | 29445 | 0.66 | 0.630717 |
Target: 5'- uGCGCGucGAuuuagucgUCUGUCCcGGUUCGGCGAa -3' miRNA: 3'- cCGCGU--CU--------GGGCAGGcUCAAGUCGCU- -5' |
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22573 | 5' | -57.4 | NC_005091.1 | + | 19545 | 0.67 | 0.598591 |
Target: 5'- cGcCGCAGGCCg--UCGuGUUCGGCGAu -3' miRNA: 3'- cC-GCGUCUGGgcaGGCuCAAGUCGCU- -5' |
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22573 | 5' | -57.4 | NC_005091.1 | + | 10377 | 0.67 | 0.577295 |
Target: 5'- cGGCGCGGAUaCCG-CC---UUCGGCGAc -3' miRNA: 3'- -CCGCGUCUG-GGCaGGcucAAGUCGCU- -5' |
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22573 | 5' | -57.4 | NC_005091.1 | + | 18324 | 0.67 | 0.545691 |
Target: 5'- cGCGCGGACCUgcuuGUCCucG-UCGGUGAu -3' miRNA: 3'- cCGCGUCUGGG----CAGGcuCaAGUCGCU- -5' |
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22573 | 5' | -57.4 | NC_005091.1 | + | 35567 | 0.68 | 0.514663 |
Target: 5'- aGCGCAGugUCuuucaauagGUUCGAGUUCAG-GAa -3' miRNA: 3'- cCGCGUCugGG---------CAGGCUCAAGUCgCU- -5' |
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22573 | 5' | -57.4 | NC_005091.1 | + | 24813 | 0.68 | 0.484376 |
Target: 5'- uGCGCAGGCCg--CCGAGgaagccguucUUCAGCGu -3' miRNA: 3'- cCGCGUCUGGgcaGGCUC----------AAGUCGCu -5' |
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22573 | 5' | -57.4 | NC_005091.1 | + | 42650 | 0.69 | 0.47447 |
Target: 5'- cGGCGCGGACaagCGUCUuc--UCGGCGAg -3' miRNA: 3'- -CCGCGUCUGg--GCAGGcucaAGUCGCU- -5' |
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22573 | 5' | -57.4 | NC_005091.1 | + | 40882 | 0.69 | 0.449204 |
Target: 5'- cGUGCAGAgcgcccagucguuguCgCCGUCCG-GUUCGGCGc -3' miRNA: 3'- cCGCGUCU---------------G-GGCAGGCuCAAGUCGCu -5' |
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22573 | 5' | -57.4 | NC_005091.1 | + | 2813 | 0.69 | 0.445382 |
Target: 5'- cGCGagAGAUaUCGUCCGGGUcaUCGGCGAa -3' miRNA: 3'- cCGCg-UCUG-GGCAGGCUCA--AGUCGCU- -5' |
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22573 | 5' | -57.4 | NC_005091.1 | + | 43651 | 0.7 | 0.426554 |
Target: 5'- aGGUGCAgaaGACCUGUCCGGcGcUUCucGCGAu -3' miRNA: 3'- -CCGCGU---CUGGGCAGGCU-C-AAGu-CGCU- -5' |
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22573 | 5' | -57.4 | NC_005091.1 | + | 13953 | 0.7 | 0.381632 |
Target: 5'- cGGUGCGGACUCGggggUCGAGUgauacGCGAu -3' miRNA: 3'- -CCGCGUCUGGGCa---GGCUCAagu--CGCU- -5' |
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22573 | 5' | -57.4 | NC_005091.1 | + | 32178 | 0.74 | 0.247555 |
Target: 5'- cGGCGCGGACCCGgCCGg---CGGCa- -3' miRNA: 3'- -CCGCGUCUGGGCaGGCucaaGUCGcu -5' |
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22573 | 5' | -57.4 | NC_005091.1 | + | 17566 | 1.11 | 0.000524 |
Target: 5'- cGGCGCAGACCCGUCCGAGUUCAGCGAa -3' miRNA: 3'- -CCGCGUCUGGGCAGGCUCAAGUCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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