Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22574 | 3' | -53.6 | NC_005091.1 | + | 19935 | 0.66 | 0.854726 |
Target: 5'- gGCGGCuucaugaaGUUcGAGCGcGAGCCGGg -3' miRNA: 3'- gUGCUGcaug----CAAcUUCGC-CUCGGCC- -5' |
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22574 | 3' | -53.6 | NC_005091.1 | + | 25073 | 0.66 | 0.851377 |
Target: 5'- gCGCGGCGUucaaccgaucgGCGgccacgGAGGCGGucgccaucgacgcGCCGGc -3' miRNA: 3'- -GUGCUGCA-----------UGCaa----CUUCGCCu------------CGGCC- -5' |
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22574 | 3' | -53.6 | NC_005091.1 | + | 52094 | 0.66 | 0.846286 |
Target: 5'- gCGCGGcCGUAUGccgUGAaaaGGCGGccgucGCCGGc -3' miRNA: 3'- -GUGCU-GCAUGCa--ACU---UCGCCu----CGGCC- -5' |
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22574 | 3' | -53.6 | NC_005091.1 | + | 17308 | 0.66 | 0.846286 |
Target: 5'- cCACGAag-ACGU--GAGCGucGGGCCGGa -3' miRNA: 3'- -GUGCUgcaUGCAacUUCGC--CUCGGCC- -5' |
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22574 | 3' | -53.6 | NC_005091.1 | + | 7313 | 0.66 | 0.837625 |
Target: 5'- gCACGgagaGCGUgGCGUUGAAGacCGGAacGUCGGc -3' miRNA: 3'- -GUGC----UGCA-UGCAACUUC--GCCU--CGGCC- -5' |
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22574 | 3' | -53.6 | NC_005091.1 | + | 4308 | 0.66 | 0.837625 |
Target: 5'- -cUGACGUACGgcacggUGAcgGGCGcgccGCCGGg -3' miRNA: 3'- guGCUGCAUGCa-----ACU--UCGCcu--CGGCC- -5' |
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22574 | 3' | -53.6 | NC_005091.1 | + | 16655 | 0.66 | 0.823328 |
Target: 5'- aGCGACG-GCG-UGggGUacgcacggaaagacgGGGGCUGGa -3' miRNA: 3'- gUGCUGCaUGCaACuuCG---------------CCUCGGCC- -5' |
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22574 | 3' | -53.6 | NC_005091.1 | + | 26101 | 0.66 | 0.819673 |
Target: 5'- cCACGGCGUACGUcGGcguGCaGGGCgCGa -3' miRNA: 3'- -GUGCUGCAUGCAaCUu--CGcCUCG-GCc -5' |
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22574 | 3' | -53.6 | NC_005091.1 | + | 1295 | 0.68 | 0.761482 |
Target: 5'- uCGCGACGUGCGgc-AAGCaGucgaucacGCCGGg -3' miRNA: 3'- -GUGCUGCAUGCaacUUCGcCu-------CGGCC- -5' |
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22574 | 3' | -53.6 | NC_005091.1 | + | 14088 | 0.69 | 0.687825 |
Target: 5'- -uCGACGUugcCGUccacGAAGCGGuuccGGCCGGu -3' miRNA: 3'- guGCUGCAu--GCAa---CUUCGCC----UCGGCC- -5' |
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22574 | 3' | -53.6 | NC_005091.1 | + | 34437 | 0.71 | 0.589777 |
Target: 5'- aCGCGGCGUGCaGUacGAcaAGCGcGGCCGGc -3' miRNA: 3'- -GUGCUGCAUG-CAa-CU--UCGCcUCGGCC- -5' |
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22574 | 3' | -53.6 | NC_005091.1 | + | 55603 | 0.72 | 0.525538 |
Target: 5'- gGCGGCGaucgGCGaagUGAuGCGGcaAGCCGGg -3' miRNA: 3'- gUGCUGCa---UGCa--ACUuCGCC--UCGGCC- -5' |
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22574 | 3' | -53.6 | NC_005091.1 | + | 48932 | 0.72 | 0.504686 |
Target: 5'- cUACGcuuguGCGUGCGacgguUUGAGGCGGGGCaGGa -3' miRNA: 3'- -GUGC-----UGCAUGC-----AACUUCGCCUCGgCC- -5' |
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22574 | 3' | -53.6 | NC_005091.1 | + | 18068 | 1.11 | 0.001331 |
Target: 5'- cCACGACGUACGUUGAAGCGGAGCCGGg -3' miRNA: 3'- -GUGCUGCAUGCAACUUCGCCUCGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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