Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22575 | 3' | -58.6 | NC_005091.1 | + | 9779 | 0.66 | 0.569462 |
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Target: 5'- cUGGGcggcugaaGGCCCGGUCgaugagucgCUGgcccagCCGGGa -3' miRNA: 3'- -ACCCug------CCGGGCCAGaa-------GAUa-----GGCCU- -5' |
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| 22575 | 3' | -58.6 | NC_005091.1 | + | 44155 | 0.66 | 0.558961 |
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Target: 5'- cGGGGCGuaugCCGGUCUUCaaccacGUCuCGGAu -3' miRNA: 3'- aCCCUGCcg--GGCCAGAAGa-----UAG-GCCU- -5' |
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| 22575 | 3' | -58.6 | NC_005091.1 | + | 15262 | 0.66 | 0.548517 |
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Target: 5'- cGGGucaacgcacGCGG-CCGGUCUcgUCcccgAUCCGGGc -3' miRNA: 3'- aCCC---------UGCCgGGCCAGA--AGa---UAGGCCU- -5' |
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| 22575 | 3' | -58.6 | NC_005091.1 | + | 5111 | 0.67 | 0.477472 |
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Target: 5'- aUGGG-CGGCCCGuaCUUCU-UCCGuGAc -3' miRNA: 3'- -ACCCuGCCGGGCcaGAAGAuAGGC-CU- -5' |
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| 22575 | 3' | -58.6 | NC_005091.1 | + | 1275 | 0.68 | 0.448426 |
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Target: 5'- cGGGAUGGCCgGGUucgcCUUCaggaaauccauuUcgCCGGGa -3' miRNA: 3'- aCCCUGCCGGgCCA----GAAG------------AuaGGCCU- -5' |
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| 22575 | 3' | -58.6 | NC_005091.1 | + | 48613 | 0.68 | 0.438962 |
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Target: 5'- cGGGGCGGCCguCGGgg-UCccAUCCGGc -3' miRNA: 3'- aCCCUGCCGG--GCCagaAGa-UAGGCCu -5' |
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| 22575 | 3' | -58.6 | NC_005091.1 | + | 18852 | 1.09 | 0.000563 |
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Target: 5'- aUGGGACGGCCCGGUCUUCUAUCCGGAg -3' miRNA: 3'- -ACCCUGCCGGGCCAGAAGAUAGGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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