Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22576 | 3' | -51.8 | NC_005091.1 | + | 51578 | 0.66 | 0.883456 |
Target: 5'- gUCGUgcgUCGCGugAcggGCgcgcagaaGCGCGGCa -3' miRNA: 3'- aAGCAa--AGCGCugUa--CGa-------CGCGUUGc -5' |
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22576 | 3' | -51.8 | NC_005091.1 | + | 9085 | 0.66 | 0.883456 |
Target: 5'- -cCGUgggUCgGCGuCGUGCUgaaGCGCAACc -3' miRNA: 3'- aaGCAa--AG-CGCuGUACGA---CGCGUUGc -5' |
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22576 | 3' | -51.8 | NC_005091.1 | + | 18307 | 0.66 | 0.883456 |
Target: 5'- -aUGUUUC-CGACGUuCUGCGCG-CGg -3' miRNA: 3'- aaGCAAAGcGCUGUAcGACGCGUuGC- -5' |
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22576 | 3' | -51.8 | NC_005091.1 | + | 4848 | 0.66 | 0.875586 |
Target: 5'- -aCGUUUUGCG-CGUGCagUGaCGUGGCGa -3' miRNA: 3'- aaGCAAAGCGCuGUACG--AC-GCGUUGC- -5' |
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22576 | 3' | -51.8 | NC_005091.1 | + | 33428 | 0.67 | 0.832381 |
Target: 5'- -gCGUccUCGCGAgCGcGCUGCGCGAa- -3' miRNA: 3'- aaGCAa-AGCGCU-GUaCGACGCGUUgc -5' |
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22576 | 3' | -51.8 | NC_005091.1 | + | 42057 | 0.67 | 0.832381 |
Target: 5'- -gCGUgccacgGCGACGUGCUGCuCGACc -3' miRNA: 3'- aaGCAaag---CGCUGUACGACGcGUUGc -5' |
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22576 | 3' | -51.8 | NC_005091.1 | + | 42797 | 0.67 | 0.832381 |
Target: 5'- ------gCGCGACGagaUGCUGCGCGAg- -3' miRNA: 3'- aagcaaaGCGCUGU---ACGACGCGUUgc -5' |
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22576 | 3' | -51.8 | NC_005091.1 | + | 30907 | 0.67 | 0.813464 |
Target: 5'- aUCGUcagUCGCGugauCGUGCUGUuCGACu -3' miRNA: 3'- aAGCAa--AGCGCu---GUACGACGcGUUGc -5' |
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22576 | 3' | -51.8 | NC_005091.1 | + | 11390 | 0.67 | 0.803696 |
Target: 5'- gUCGgc-CGCGuCAcGCUGCGCuuCGg -3' miRNA: 3'- aAGCaaaGCGCuGUaCGACGCGuuGC- -5' |
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22576 | 3' | -51.8 | NC_005091.1 | + | 1288 | 0.67 | 0.803696 |
Target: 5'- -gUGUucUUCGCGACGUGCgGCaaGCAGuCGa -3' miRNA: 3'- aaGCA--AAGCGCUGUACGaCG--CGUU-GC- -5' |
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22576 | 3' | -51.8 | NC_005091.1 | + | 48361 | 0.68 | 0.793736 |
Target: 5'- cUCG-UUCGgGAUAUcugcGUUGCGCAACu -3' miRNA: 3'- aAGCaAAGCgCUGUA----CGACGCGUUGc -5' |
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22576 | 3' | -51.8 | NC_005091.1 | + | 19534 | 0.68 | 0.793736 |
Target: 5'- gUCGUgUUCgGCGAU-UGCUgGUGCAGCGa -3' miRNA: 3'- aAGCA-AAG-CGCUGuACGA-CGCGUUGC- -5' |
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22576 | 3' | -51.8 | NC_005091.1 | + | 55928 | 0.68 | 0.773291 |
Target: 5'- cUCGUgcgccagUUGCuGACcgGCUGCGCcGCu -3' miRNA: 3'- aAGCAa------AGCG-CUGuaCGACGCGuUGc -5' |
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22576 | 3' | -51.8 | NC_005091.1 | + | 1821 | 0.68 | 0.752225 |
Target: 5'- gUCGaccgUCGCGACGUGCUccuuCGCuuCGg -3' miRNA: 3'- aAGCaa--AGCGCUGUACGAc---GCGuuGC- -5' |
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22576 | 3' | -51.8 | NC_005091.1 | + | 13314 | 0.69 | 0.741491 |
Target: 5'- gUUCGUUgCGCGGaucGCgGCGCAAUGg -3' miRNA: 3'- -AAGCAAaGCGCUguaCGaCGCGUUGC- -5' |
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22576 | 3' | -51.8 | NC_005091.1 | + | 51421 | 0.69 | 0.741491 |
Target: 5'- cUUGaaUCGCGAC-UGCacGCGCAGCGu -3' miRNA: 3'- aAGCaaAGCGCUGuACGa-CGCGUUGC- -5' |
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22576 | 3' | -51.8 | NC_005091.1 | + | 21756 | 0.69 | 0.719682 |
Target: 5'- -cCGUguaUUUGCGGCAgcgaaUGCgGCGCAugGg -3' miRNA: 3'- aaGCA---AAGCGCUGU-----ACGaCGCGUugC- -5' |
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22576 | 3' | -51.8 | NC_005091.1 | + | 50257 | 0.69 | 0.719682 |
Target: 5'- gUCGauUUUCGCGACcgGCauUGCGGCGa -3' miRNA: 3'- aAGC--AAAGCGCUGuaCGacGCGUUGC- -5' |
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22576 | 3' | -51.8 | NC_005091.1 | + | 17244 | 0.69 | 0.697503 |
Target: 5'- cUUCGUgg-GCGACcaguuugucuUGCUGCGCGAUGc -3' miRNA: 3'- -AAGCAaagCGCUGu---------ACGACGCGUUGC- -5' |
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22576 | 3' | -51.8 | NC_005091.1 | + | 50676 | 0.7 | 0.641086 |
Target: 5'- -aCGUcuauaaCGCGACGcUGUUGCGCGACu -3' miRNA: 3'- aaGCAaa----GCGCUGU-ACGACGCGUUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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