Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22578 | 5' | -48.2 | NC_005091.1 | + | 29244 | 0.69 | 0.926518 |
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Target: 5'- --gCCCGUauagcagguagacGUuguUCGACAGCUUcGUCGCg -3' miRNA: 3'- caaGGGCG-------------UA---AGCUGUUGAGuUAGCG- -5' |
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| 22578 | 5' | -48.2 | NC_005091.1 | + | 49817 | 0.69 | 0.907861 |
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Target: 5'- -aUCgCCGCGUUCGugGCGGCUUGcgCGUu -3' miRNA: 3'- caAG-GGCGUAAGC--UGUUGAGUuaGCG- -5' |
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| 22578 | 5' | -48.2 | NC_005091.1 | + | 25681 | 0.7 | 0.900845 |
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Target: 5'- --aCCUGCccuUUCGACAucaucugUUCGAUCGCg -3' miRNA: 3'- caaGGGCGu--AAGCUGUu------GAGUUAGCG- -5' |
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| 22578 | 5' | -48.2 | NC_005091.1 | + | 43068 | 0.71 | 0.852779 |
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Target: 5'- -cUCCCGCGUgucugCGACAggaGCcaaGGUCGCc -3' miRNA: 3'- caAGGGCGUAa----GCUGU---UGag-UUAGCG- -5' |
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| 22578 | 5' | -48.2 | NC_005091.1 | + | 843 | 0.71 | 0.838355 |
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Target: 5'- -aUCCUGCGgccaugaaauaccCGGC-GCUCAAUCGCa -3' miRNA: 3'- caAGGGCGUaa-----------GCUGuUGAGUUAGCG- -5' |
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| 22578 | 5' | -48.2 | NC_005091.1 | + | 1032 | 0.71 | 0.825236 |
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Target: 5'- gGUUCCgGCA--CGACGGCUUccgGGUCGUa -3' miRNA: 3'- -CAAGGgCGUaaGCUGUUGAG---UUAGCG- -5' |
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| 22578 | 5' | -48.2 | NC_005091.1 | + | 28551 | 0.73 | 0.742919 |
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Target: 5'- --aCCgGCGaacuguacUUCGGCAACUCGccGUCGCu -3' miRNA: 3'- caaGGgCGU--------AAGCUGUUGAGU--UAGCG- -5' |
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| 22578 | 5' | -48.2 | NC_005091.1 | + | 39480 | 0.74 | 0.697284 |
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Target: 5'- -gUCCaCGCAUUUGuccugauacgcauGCAGCUCGAUCGg -3' miRNA: 3'- caAGG-GCGUAAGC-------------UGUUGAGUUAGCg -5' |
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| 22578 | 5' | -48.2 | NC_005091.1 | + | 21101 | 1.14 | 0.002696 |
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Target: 5'- cGUUCCCGCAUUCGACAACUCAAUCGCa -3' miRNA: 3'- -CAAGGGCGUAAGCUGUUGAGUUAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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