Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22579 | 5' | -56.7 | NC_005091.1 | + | 26287 | 0.7 | 0.354967 |
Target: 5'- cGUUCGCAucguucACCgcaGCCUGCGCaGCAGc- -3' miRNA: 3'- aCGAGCGU------UGG---CGGACGCGaCGUUaa -5' |
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22579 | 5' | -56.7 | NC_005091.1 | + | 23101 | 0.74 | 0.185584 |
Target: 5'- cUGCUUG--GCgGCCUGCGCUGCAu-- -3' miRNA: 3'- -ACGAGCguUGgCGGACGCGACGUuaa -5' |
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22579 | 5' | -56.7 | NC_005091.1 | + | 21306 | 1.05 | 0.001108 |
Target: 5'- cUGCUCGCAACCGCCUGCGCUGCAAUUc -3' miRNA: 3'- -ACGAGCGUUGGCGGACGCGACGUUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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