Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22580 | 3' | -55.5 | NC_005091.1 | + | 9371 | 0.66 | 0.71765 |
Target: 5'- -cACGGCcagccGGUCggcgGCCAGCGGCg -3' miRNA: 3'- caUGCUGuaguaCCAG----CGGUCGCCGa -5' |
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22580 | 3' | -55.5 | NC_005091.1 | + | 43079 | 0.66 | 0.6852 |
Target: 5'- cUGCGACAggagcCAaGGUCGCCuugccCGGCg -3' miRNA: 3'- cAUGCUGUa----GUaCCAGCGGuc---GCCGa -5' |
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22580 | 3' | -55.5 | NC_005091.1 | + | 30790 | 0.66 | 0.6852 |
Target: 5'- -gGCGAacCGUCGUccaGUCGCgAGCGGUUg -3' miRNA: 3'- caUGCU--GUAGUAc--CAGCGgUCGCCGA- -5' |
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22580 | 3' | -55.5 | NC_005091.1 | + | 8946 | 0.67 | 0.652289 |
Target: 5'- cGUACGACGuUCA---UCGCCAGCGcaGCa -3' miRNA: 3'- -CAUGCUGU-AGUaccAGCGGUCGC--CGa -5' |
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22580 | 3' | -55.5 | NC_005091.1 | + | 34778 | 0.67 | 0.652289 |
Target: 5'- -cGCGACAgcaUCGUGGgCGCgCAGUaccGGCUc -3' miRNA: 3'- caUGCUGU---AGUACCaGCG-GUCG---CCGA- -5' |
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22580 | 3' | -55.5 | NC_005091.1 | + | 32666 | 0.67 | 0.641268 |
Target: 5'- cGU-CGACcUUGUGGUCGCCGGCa--- -3' miRNA: 3'- -CAuGCUGuAGUACCAGCGGUCGccga -5' |
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22580 | 3' | -55.5 | NC_005091.1 | + | 16356 | 0.67 | 0.641268 |
Target: 5'- -aGCGACGUggaaCGUGcucggcgcguucGUCGCgAGCGGCg -3' miRNA: 3'- caUGCUGUA----GUAC------------CAGCGgUCGCCGa -5' |
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22580 | 3' | -55.5 | NC_005091.1 | + | 6614 | 0.67 | 0.630238 |
Target: 5'- -cGCGGCGUCcgGGcUGCCGGCGu-- -3' miRNA: 3'- caUGCUGUAGuaCCaGCGGUCGCcga -5' |
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22580 | 3' | -55.5 | NC_005091.1 | + | 9283 | 0.68 | 0.597196 |
Target: 5'- -cGCGAU-UCG-GGUCGCCGGCuGCa -3' miRNA: 3'- caUGCUGuAGUaCCAGCGGUCGcCGa -5' |
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22580 | 3' | -55.5 | NC_005091.1 | + | 19664 | 0.68 | 0.582948 |
Target: 5'- cGUGUGGCucgugcUCAUGGUCGCgucguacuuucuguCGGCGGCg -3' miRNA: 3'- -CAUGCUGu-----AGUACCAGCG--------------GUCGCCGa -5' |
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22580 | 3' | -55.5 | NC_005091.1 | + | 11866 | 0.68 | 0.564422 |
Target: 5'- uUGCGGCAUgAacggaUGGUCGCUgaaauccgugaAGCGGUUg -3' miRNA: 3'- cAUGCUGUAgU-----ACCAGCGG-----------UCGCCGA- -5' |
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22580 | 3' | -55.5 | NC_005091.1 | + | 3479 | 0.69 | 0.521523 |
Target: 5'- -gGCGACAUCGaccagaUGGUCGUCucguuCGGCUc -3' miRNA: 3'- caUGCUGUAGU------ACCAGCGGuc---GCCGA- -5' |
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22580 | 3' | -55.5 | NC_005091.1 | + | 25917 | 0.69 | 0.490198 |
Target: 5'- -aACGACuUUcgGGUCGuucguuCCAGCGGCc -3' miRNA: 3'- caUGCUGuAGuaCCAGC------GGUCGCCGa -5' |
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22580 | 3' | -55.5 | NC_005091.1 | + | 21638 | 1.08 | 0.001036 |
Target: 5'- cGUACGACAUCAUGGUCGCCAGCGGCUa -3' miRNA: 3'- -CAUGCUGUAGUACCAGCGGUCGCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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