Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22581 | 3' | -51.1 | NC_005091.1 | + | 45331 | 0.72 | 0.617679 |
Target: 5'- aGUUCcgGGCGCaaCGCGCGGCcggUAGa- -3' miRNA: 3'- -CAAGuaCUGCGa-GCGCGCCGa--AUUag -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 30596 | 0.72 | 0.56107 |
Target: 5'- -cUCAUGACGCagagCGCGCcGCUgaUGGUCc -3' miRNA: 3'- caAGUACUGCGa---GCGCGcCGA--AUUAG- -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 32306 | 0.73 | 0.549895 |
Target: 5'- aGUUCAggaugccugcuUGGCGCUCGCGguCGGcCUUGAUa -3' miRNA: 3'- -CAAGU-----------ACUGCGAGCGC--GCC-GAAUUAg -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 30262 | 0.73 | 0.549895 |
Target: 5'- --cCGUGGCGCUCGUGCagauGCUcGAUCu -3' miRNA: 3'- caaGUACUGCGAGCGCGc---CGAaUUAG- -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 23505 | 0.74 | 0.495221 |
Target: 5'- -aUCAUGGCGacgCGCGCGGCg----- -3' miRNA: 3'- caAGUACUGCga-GCGCGCCGaauuag -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 6599 | 0.75 | 0.423197 |
Target: 5'- -gUCgAUGAuCGCUCGCGCGGCg----- -3' miRNA: 3'- caAG-UACU-GCGAGCGCGCCGaauuag -5' |
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22581 | 3' | -51.1 | NC_005091.1 | + | 21923 | 1.11 | 0.001774 |
Target: 5'- cGUUCAUGACGCUCGCGCGGCUUAAUCa -3' miRNA: 3'- -CAAGUACUGCGAGCGCGCCGAAUUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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