Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22581 | 5' | -50.7 | NC_005091.1 | + | 1045 | 0.7 | 0.733983 |
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Target: 5'- gGAGAUCAGCgCGgGUu-CCGGCACGAc -3' miRNA: 3'- -CUCUAGUUG-GUgUAcuGGCUGUGCUu -5' |
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| 22581 | 5' | -50.7 | NC_005091.1 | + | 17678 | 0.71 | 0.70068 |
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Target: 5'- -uGGUCAGCUACGacAUCGGCACGAAg -3' miRNA: 3'- cuCUAGUUGGUGUacUGGCUGUGCUU- -5' |
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| 22581 | 5' | -50.7 | NC_005091.1 | + | 24643 | 0.71 | 0.678097 |
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Target: 5'- -cGAUCGacACCGC-UGACCGAUACGu- -3' miRNA: 3'- cuCUAGU--UGGUGuACUGGCUGUGCuu -5' |
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| 22581 | 5' | -50.7 | NC_005091.1 | + | 8025 | 0.71 | 0.655325 |
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Target: 5'- cGAGGUCGGCCuGCAUuaccugucguucGACCGAC-CGGAg -3' miRNA: 3'- -CUCUAGUUGG-UGUA------------CUGGCUGuGCUU- -5' |
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| 22581 | 5' | -50.7 | NC_005091.1 | + | 11508 | 0.73 | 0.541808 |
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Target: 5'- --cGUCGGCCgACGUGGCCGcCGCGAAa -3' miRNA: 3'- cucUAGUUGG-UGUACUGGCuGUGCUU- -5' |
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| 22581 | 5' | -50.7 | NC_005091.1 | + | 28140 | 0.75 | 0.435531 |
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Target: 5'- cGGAUgCAGCCgACAUcACCGACACGAAg -3' miRNA: 3'- cUCUA-GUUGG-UGUAcUGGCUGUGCUU- -5' |
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| 22581 | 5' | -50.7 | NC_005091.1 | + | 36851 | 0.76 | 0.377822 |
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Target: 5'- -cGAUCGGCCGCGuaugccUGACCGACuACGAc -3' miRNA: 3'- cuCUAGUUGGUGU------ACUGGCUG-UGCUu -5' |
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| 22581 | 5' | -50.7 | NC_005091.1 | + | 21886 | 1.07 | 0.00394 |
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Target: 5'- aGAGAUCAACCACAUGACCGACACGAAg -3' miRNA: 3'- -CUCUAGUUGGUGUACUGGCUGUGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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