Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22582 | 3' | -51.8 | NC_005091.1 | + | 20396 | 0.66 | 0.895663 |
Target: 5'- cGAGCagGAGCGGUcGCCCGuuGAAGuuGCu -3' miRNA: 3'- -UUUG--CUUGCUAaUGGGC--CUUCggCGu -5' |
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22582 | 3' | -51.8 | NC_005091.1 | + | 56691 | 0.66 | 0.895663 |
Target: 5'- ---gGggUGAUgugGCaUCGGAGGCCGUg -3' miRNA: 3'- uuugCuuGCUAa--UG-GGCCUUCGGCGu -5' |
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22582 | 3' | -51.8 | NC_005091.1 | + | 14102 | 0.66 | 0.894936 |
Target: 5'- -cACGaAGCGGUUccggccgguaguaGCCgGGAGGUCGCc -3' miRNA: 3'- uuUGC-UUGCUAA-------------UGGgCCUUCGGCGu -5' |
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22582 | 3' | -51.8 | NC_005091.1 | + | 11876 | 0.66 | 0.885228 |
Target: 5'- cGGCGAGCGGccgaUACCaacagaugcugaaGGAcuGGCCGCAc -3' miRNA: 3'- uUUGCUUGCUa---AUGGg------------CCU--UCGGCGU- -5' |
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22582 | 3' | -51.8 | NC_005091.1 | + | 25997 | 0.66 | 0.880591 |
Target: 5'- gGAACGAACGA---CCCGaAAGUCGUu -3' miRNA: 3'- -UUUGCUUGCUaauGGGCcUUCGGCGu -5' |
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22582 | 3' | -51.8 | NC_005091.1 | + | 22819 | 0.66 | 0.872646 |
Target: 5'- cGGCGcGGCGA-UAUCCGGAGcgaacGCCGCu -3' miRNA: 3'- uUUGC-UUGCUaAUGGGCCUU-----CGGCGu -5' |
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22582 | 3' | -51.8 | NC_005091.1 | + | 2017 | 0.66 | 0.864438 |
Target: 5'- uGGCGAACGAcgAauaUGGuGAGCCGCAg -3' miRNA: 3'- uUUGCUUGCUaaUgg-GCC-UUCGGCGU- -5' |
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22582 | 3' | -51.8 | NC_005091.1 | + | 14732 | 0.66 | 0.864438 |
Target: 5'- -uGCGAGCGccguagcACCCGGA-GUCGUAg -3' miRNA: 3'- uuUGCUUGCuaa----UGGGCCUuCGGCGU- -5' |
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22582 | 3' | -51.8 | NC_005091.1 | + | 10129 | 0.67 | 0.843709 |
Target: 5'- gAGACGGACG-UUACgCCGGcacgcgccgagcGCCGCAc -3' miRNA: 3'- -UUUGCUUGCuAAUG-GGCCuu----------CGGCGU- -5' |
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22582 | 3' | -51.8 | NC_005091.1 | + | 10316 | 0.67 | 0.83831 |
Target: 5'- cGAGCGGucguACGAUUuuCCCGGAAGaguaCUGCGc -3' miRNA: 3'- -UUUGCU----UGCUAAu-GGGCCUUC----GGCGU- -5' |
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22582 | 3' | -51.8 | NC_005091.1 | + | 52597 | 0.67 | 0.832829 |
Target: 5'- cGGACGAagcAUGAgaacaGCCCGGAaucccacaagccgacAGCCGCc -3' miRNA: 3'- -UUUGCU---UGCUaa---UGGGCCU---------------UCGGCGu -5' |
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22582 | 3' | -51.8 | NC_005091.1 | + | 34069 | 0.67 | 0.829129 |
Target: 5'- --uCGGGCGGcaGCUCGGAcucgauagcGGCCGCGu -3' miRNA: 3'- uuuGCUUGCUaaUGGGCCU---------UCGGCGU- -5' |
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22582 | 3' | -51.8 | NC_005091.1 | + | 8899 | 0.67 | 0.819729 |
Target: 5'- cAAGCGGAucacCGAagACCCGuacGAAGCUGCGg -3' miRNA: 3'- -UUUGCUU----GCUaaUGGGC---CUUCGGCGU- -5' |
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22582 | 3' | -51.8 | NC_005091.1 | + | 10729 | 0.67 | 0.819729 |
Target: 5'- -cACGAGcCGAU--CCCGaGAAGCCgGCAu -3' miRNA: 3'- uuUGCUU-GCUAauGGGC-CUUCGG-CGU- -5' |
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22582 | 3' | -51.8 | NC_005091.1 | + | 50720 | 0.67 | 0.810121 |
Target: 5'- cGAGCgGAACGAcgaaCCGGccGCCGCGu -3' miRNA: 3'- -UUUG-CUUGCUaaugGGCCuuCGGCGU- -5' |
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22582 | 3' | -51.8 | NC_005091.1 | + | 941 | 0.68 | 0.759347 |
Target: 5'- -uGCGGauGCGAUUgaGCgCCGGGuauuucauGGCCGCAg -3' miRNA: 3'- uuUGCU--UGCUAA--UG-GGCCU--------UCGGCGU- -5' |
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22582 | 3' | -51.8 | NC_005091.1 | + | 57531 | 0.69 | 0.737987 |
Target: 5'- cGAAUGGACGcccgGCCCGGAuucGCCGa- -3' miRNA: 3'- -UUUGCUUGCuaa-UGGGCCUu--CGGCgu -5' |
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22582 | 3' | -51.8 | NC_005091.1 | + | 9367 | 0.69 | 0.737987 |
Target: 5'- cGACGG--GAcUAUCCGGAGGCCGUu -3' miRNA: 3'- uUUGCUugCUaAUGGGCCUUCGGCGu -5' |
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22582 | 3' | -51.8 | NC_005091.1 | + | 137 | 0.69 | 0.737987 |
Target: 5'- cGAAUGGACGcccgGCCCGGAuucGCCGa- -3' miRNA: 3'- -UUUGCUUGCuaa-UGGGCCUu--CGGCgu -5' |
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22582 | 3' | -51.8 | NC_005091.1 | + | 20922 | 0.69 | 0.727132 |
Target: 5'- aAGGCGGAC-AUUACCCGGcAGGCgaCGUAa -3' miRNA: 3'- -UUUGCUUGcUAAUGGGCC-UUCG--GCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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