Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22582 | 5' | -53.6 | NC_005091.1 | + | 6761 | 0.66 | 0.791899 |
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Target: 5'- gGUCGCGGaaGUcCCGGUGU-UCGUg-- -3' miRNA: 3'- -UAGCGCCgaUA-GGCCACAaAGCAgaa -5' |
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| 22582 | 5' | -53.6 | NC_005091.1 | + | 11582 | 0.66 | 0.781811 |
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Target: 5'- cAUCGCGGCUAUCgGGgcugcuaUCGUa-- -3' miRNA: 3'- -UAGCGCCGAUAGgCCacaa---AGCAgaa -5' |
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| 22582 | 5' | -53.6 | NC_005091.1 | + | 50635 | 0.66 | 0.750619 |
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Target: 5'- -aCGCGGCgg-CCGGU---UCGUCg- -3' miRNA: 3'- uaGCGCCGauaGGCCAcaaAGCAGaa -5' |
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| 22582 | 5' | -53.6 | NC_005091.1 | + | 27498 | 0.67 | 0.729174 |
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Target: 5'- aGUCGuCGGCUuUCCGGUcugccUCGUCg- -3' miRNA: 3'- -UAGC-GCCGAuAGGCCAcaa--AGCAGaa -5' |
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| 22582 | 5' | -53.6 | NC_005091.1 | + | 29452 | 0.68 | 0.640293 |
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Target: 5'- -gCGCGGCUuUCUGGUG---CGUCUg -3' miRNA: 3'- uaGCGCCGAuAGGCCACaaaGCAGAa -5' |
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| 22582 | 5' | -53.6 | NC_005091.1 | + | 22427 | 1.03 | 0.003647 |
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Target: 5'- uAUCGCGGCUAUCCGGUGUUUCGUCUUc -3' miRNA: 3'- -UAGCGCCGAUAGGCCACAAAGCAGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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