Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22583 | 3' | -50.8 | NC_005091.1 | + | 47025 | 0.66 | 0.928845 |
Target: 5'- cGCAgaagcGGCCGAAuuGGGCGaucguGUUGCa -3' miRNA: 3'- cCGUa----CCGGCUU--UCCGUgcuu-CAAUG- -5' |
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22583 | 3' | -50.8 | NC_005091.1 | + | 13769 | 0.66 | 0.922884 |
Target: 5'- -aCGUGGUCGGucGGCACGAc----- -3' miRNA: 3'- ccGUACCGGCUuuCCGUGCUucaaug -5' |
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22583 | 3' | -50.8 | NC_005091.1 | + | 30252 | 0.66 | 0.916628 |
Target: 5'- cGGCGacaaGGCCG--GGGCGuCGAAGccgGCg -3' miRNA: 3'- -CCGUa---CCGGCuuUCCGU-GCUUCaa-UG- -5' |
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22583 | 3' | -50.8 | NC_005091.1 | + | 43531 | 0.66 | 0.910078 |
Target: 5'- uGCGcGG-CGAAuuGaGCACGAAGUUACu -3' miRNA: 3'- cCGUaCCgGCUUu-C-CGUGCUUCAAUG- -5' |
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22583 | 3' | -50.8 | NC_005091.1 | + | 11851 | 0.66 | 0.903236 |
Target: 5'- cGGCuuacGGCCGAAuugcGGCAUGAacggauGGUcGCu -3' miRNA: 3'- -CCGua--CCGGCUUu---CCGUGCU------UCAaUG- -5' |
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22583 | 3' | -50.8 | NC_005091.1 | + | 6171 | 0.67 | 0.888692 |
Target: 5'- gGGCGacgaGCCGAAGcGGCGCGAcAGUcggACg -3' miRNA: 3'- -CCGUac--CGGCUUU-CCGUGCU-UCAa--UG- -5' |
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22583 | 3' | -50.8 | NC_005091.1 | + | 7655 | 0.67 | 0.888692 |
Target: 5'- aGGCc--GCCGAAGcGGCccUGAAGUUACu -3' miRNA: 3'- -CCGuacCGGCUUU-CCGu-GCUUCAAUG- -5' |
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22583 | 3' | -50.8 | NC_005091.1 | + | 52115 | 0.67 | 0.880999 |
Target: 5'- aGGC--GGCCGucgccGGCGCGggGcgcgUUGCg -3' miRNA: 3'- -CCGuaCCGGCuuu--CCGUGCuuC----AAUG- -5' |
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22583 | 3' | -50.8 | NC_005091.1 | + | 23836 | 0.67 | 0.880999 |
Target: 5'- uGCGUaaucGcGCCGAGcuGCACGAGGUUGu -3' miRNA: 3'- cCGUA----C-CGGCUUucCGUGCUUCAAUg -5' |
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22583 | 3' | -50.8 | NC_005091.1 | + | 7543 | 0.67 | 0.873032 |
Target: 5'- cGGCAcGGCgGAAGGGCuguaaaucgGAAGUaACu -3' miRNA: 3'- -CCGUaCCGgCUUUCCGug-------CUUCAaUG- -5' |
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22583 | 3' | -50.8 | NC_005091.1 | + | 17716 | 0.67 | 0.8648 |
Target: 5'- gGGCAUGGCCGcGAuuaucGGCGacgagaucauCGAGGUg-- -3' miRNA: 3'- -CCGUACCGGCuUU-----CCGU----------GCUUCAaug -5' |
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22583 | 3' | -50.8 | NC_005091.1 | + | 52712 | 0.68 | 0.838583 |
Target: 5'- cGGCuuUGcGCUggGAAAGGCGCGGAuGUUGa -3' miRNA: 3'- -CCGu-AC-CGG--CUUUCCGUGCUU-CAAUg -5' |
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22583 | 3' | -50.8 | NC_005091.1 | + | 4683 | 0.69 | 0.810284 |
Target: 5'- gGGCcggGGCCGucgcuGGUagcuccccggacACGAAGUUACu -3' miRNA: 3'- -CCGua-CCGGCuuu--CCG------------UGCUUCAAUG- -5' |
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22583 | 3' | -50.8 | NC_005091.1 | + | 1688 | 0.7 | 0.748626 |
Target: 5'- cGGCAUcggcuaucGGCCuGAAGGaGCGCGucaaaGGGUUACu -3' miRNA: 3'- -CCGUA--------CCGG-CUUUC-CGUGC-----UUCAAUG- -5' |
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22583 | 3' | -50.8 | NC_005091.1 | + | 15539 | 0.71 | 0.67114 |
Target: 5'- cGGCAUGGaCUGucacGGGUGCGggGccgUACa -3' miRNA: 3'- -CCGUACC-GGCuu--UCCGUGCuuCa--AUG- -5' |
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22583 | 3' | -50.8 | NC_005091.1 | + | 48589 | 0.72 | 0.648465 |
Target: 5'- cGGCGaGGCgCGAAuGGCGgGAGGgcGCa -3' miRNA: 3'- -CCGUaCCG-GCUUuCCGUgCUUCaaUG- -5' |
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22583 | 3' | -50.8 | NC_005091.1 | + | 44869 | 0.8 | 0.25497 |
Target: 5'- cGGCAaggcGGCCG-AAGGCACGAAGgcaACg -3' miRNA: 3'- -CCGUa---CCGGCuUUCCGUGCUUCaa-UG- -5' |
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22583 | 3' | -50.8 | NC_005091.1 | + | 22585 | 1.13 | 0.001633 |
Target: 5'- aGGCAUGGCCGAAAGGCACGAAGUUACu -3' miRNA: 3'- -CCGUACCGGCUUUCCGUGCUUCAAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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