Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22585 | 3' | -51 | NC_005091.1 | + | 15734 | 0.66 | 0.92321 |
Target: 5'- gCGAUCUGCGCGcCAUUCguaucgaUGAACCGc- -3' miRNA: 3'- -GUUAGGCGCGUuGUAGG-------ACUUGGUcu -5' |
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22585 | 3' | -51 | NC_005091.1 | + | 55941 | 0.66 | 0.911112 |
Target: 5'- uCAggCCGUGCAGC-UCgUGcGCCAGu -3' miRNA: 3'- -GUuaGGCGCGUUGuAGgACuUGGUCu -5' |
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22585 | 3' | -51 | NC_005091.1 | + | 23842 | 0.67 | 0.889876 |
Target: 5'- aGAUUCaGCGCAugGUCgCUGAGaugCAGAa -3' miRNA: 3'- gUUAGG-CGCGUugUAG-GACUUg--GUCU- -5' |
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22585 | 3' | -51 | NC_005091.1 | + | 22172 | 0.67 | 0.882232 |
Target: 5'- gCAAUCCGCGCAggucgGCAUUuugCUGGcgGCCGu- -3' miRNA: 3'- -GUUAGGCGCGU-----UGUAG---GACU--UGGUcu -5' |
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22585 | 3' | -51 | NC_005091.1 | + | 32392 | 0.67 | 0.882232 |
Target: 5'- ---aCCGCGagcgccaagcaGGCAUCCUGAACUgcgaAGAa -3' miRNA: 3'- guuaGGCGCg----------UUGUAGGACUUGG----UCU- -5' |
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22585 | 3' | -51 | NC_005091.1 | + | 22461 | 0.67 | 0.874314 |
Target: 5'- -cAUCUGCGgcuggucgguCAGCAuggcuUCCUGAAUCGGAa -3' miRNA: 3'- guUAGGCGC----------GUUGU-----AGGACUUGGUCU- -5' |
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22585 | 3' | -51 | NC_005091.1 | + | 36053 | 0.67 | 0.874314 |
Target: 5'- gCAAUCCGUaCGACAUCUaugucgUGGACCGc- -3' miRNA: 3'- -GUUAGGCGcGUUGUAGG------ACUUGGUcu -5' |
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22585 | 3' | -51 | NC_005091.1 | + | 2112 | 0.67 | 0.866129 |
Target: 5'- gAGUCCGCGCAACuga-UGGuGCCGGu -3' miRNA: 3'- gUUAGGCGCGUUGuaggACU-UGGUCu -5' |
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22585 | 3' | -51 | NC_005091.1 | + | 19338 | 0.67 | 0.866129 |
Target: 5'- ---gCUGCGCAACggCCUGAagAUCAGc -3' miRNA: 3'- guuaGGCGCGUUGuaGGACU--UGGUCu -5' |
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22585 | 3' | -51 | NC_005091.1 | + | 34389 | 0.68 | 0.830879 |
Target: 5'- uCAggUCGCGCAccccgGCGaCCUGuACCAGAu -3' miRNA: 3'- -GUuaGGCGCGU-----UGUaGGACuUGGUCU- -5' |
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22585 | 3' | -51 | NC_005091.1 | + | 13240 | 0.68 | 0.821485 |
Target: 5'- gCGAUCCGCGCAACGaaCUUGuuGGCCGc- -3' miRNA: 3'- -GUUAGGCGCGUUGUa-GGAC--UUGGUcu -5' |
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22585 | 3' | -51 | NC_005091.1 | + | 21846 | 0.69 | 0.792073 |
Target: 5'- ---gCCGCGCgAGCGUCaUGAACgAGAc -3' miRNA: 3'- guuaGGCGCG-UUGUAGgACUUGgUCU- -5' |
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22585 | 3' | -51 | NC_005091.1 | + | 6604 | 0.69 | 0.761064 |
Target: 5'- uGAUCgcuCGCGCGGCGUCCgGGcuGCCGGc -3' miRNA: 3'- gUUAG---GCGCGUUGUAGGaCU--UGGUCu -5' |
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22585 | 3' | -51 | NC_005091.1 | + | 3219 | 0.71 | 0.684368 |
Target: 5'- aCAGgaugUCGUGCAccGCGUCCUGAagcucuucgaggACCGGAu -3' miRNA: 3'- -GUUa---GGCGCGU--UGUAGGACU------------UGGUCU- -5' |
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22585 | 3' | -51 | NC_005091.1 | + | 23554 | 0.71 | 0.673099 |
Target: 5'- aCAGUUCGCuGC-GCAguUCCUGcGCCAGAu -3' miRNA: 3'- -GUUAGGCG-CGuUGU--AGGACuUGGUCU- -5' |
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22585 | 3' | -51 | NC_005091.1 | + | 51555 | 0.72 | 0.605025 |
Target: 5'- gCGAUCCGCGCGGCAUCgCcaagUGGgcggaagcgacgACCGGGg -3' miRNA: 3'- -GUUAGGCGCGUUGUAG-G----ACU------------UGGUCU- -5' |
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22585 | 3' | -51 | NC_005091.1 | + | 2707 | 0.74 | 0.483827 |
Target: 5'- aUAGUCUGCGCcguaucgaacGCAUCCgcaGAACCGGAg -3' miRNA: 3'- -GUUAGGCGCGu---------UGUAGGa--CUUGGUCU- -5' |
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22585 | 3' | -51 | NC_005091.1 | + | 4667 | 0.76 | 0.375493 |
Target: 5'- --cUCCGCGCGuCGUUUUGGGCCGGGg -3' miRNA: 3'- guuAGGCGCGUuGUAGGACUUGGUCU- -5' |
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22585 | 3' | -51 | NC_005091.1 | + | 23216 | 0.99 | 0.014246 |
Target: 5'- aCAAUCCGCGCAACAUCC-GAACCAGAc -3' miRNA: 3'- -GUUAGGCGCGUUGUAGGaCUUGGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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