Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22588 | 3' | -51.8 | NC_005091.1 | + | 12971 | 0.66 | 0.889341 |
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Target: 5'- --cCACGGUCGUCCgggccGAGCGgcguauugCCUg- -3' miRNA: 3'- caaGUGCCAGCAGGa----CUUGUa-------GGAgc -5' |
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| 22588 | 3' | -51.8 | NC_005091.1 | + | 55355 | 0.66 | 0.889341 |
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Target: 5'- gGUUUcgGCGGU-GUCCaUGGugAUCCUCc -3' miRNA: 3'- -CAAG--UGCCAgCAGG-ACUugUAGGAGc -5' |
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| 22588 | 3' | -51.8 | NC_005091.1 | + | 57454 | 0.67 | 0.874033 |
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Target: 5'- uUUCAUGGcCGcCCgcuucGAGCAUCUUCa -3' miRNA: 3'- cAAGUGCCaGCaGGa----CUUGUAGGAGc -5' |
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| 22588 | 3' | -51.8 | NC_005091.1 | + | 13188 | 0.67 | 0.865989 |
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Target: 5'- aGUUCAaagGGUCGUCCccGGACGUUCgggaCGa -3' miRNA: 3'- -CAAGUg--CCAGCAGGa-CUUGUAGGa---GC- -5' |
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| 22588 | 3' | -51.8 | NC_005091.1 | + | 11938 | 0.69 | 0.738843 |
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Target: 5'- aGUUCACGaggaugucguagccGUCGUCCUGcgugacgguGACGUgCUCGu -3' miRNA: 3'- -CAAGUGC--------------CAGCAGGAC---------UUGUAgGAGC- -5' |
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| 22588 | 3' | -51.8 | NC_005091.1 | + | 13078 | 0.7 | 0.731371 |
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Target: 5'- -aUCGCGGuacuggacaacaUCGUCCcGAACGUCCg-- -3' miRNA: 3'- caAGUGCC------------AGCAGGaCUUGUAGGagc -5' |
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| 22588 | 3' | -51.8 | NC_005091.1 | + | 18102 | 0.7 | 0.698835 |
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Target: 5'- -aUCACGGcCGUgCaGGAUAUCUUCGg -3' miRNA: 3'- caAGUGCCaGCAgGaCUUGUAGGAGC- -5' |
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| 22588 | 3' | -51.8 | NC_005091.1 | + | 55440 | 0.71 | 0.676791 |
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Target: 5'- -gUCGCGGccgcCGUCagccGGGCGUCCUCGa -3' miRNA: 3'- caAGUGCCa---GCAGga--CUUGUAGGAGC- -5' |
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| 22588 | 3' | -51.8 | NC_005091.1 | + | 28484 | 0.71 | 0.63227 |
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Target: 5'- aGUUCGCcGGUCGUgUUGAACGUgCC-CGg -3' miRNA: 3'- -CAAGUG-CCAGCAgGACUUGUA-GGaGC- -5' |
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| 22588 | 3' | -51.8 | NC_005091.1 | + | 25378 | 1.1 | 0.002248 |
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Target: 5'- cGUUCACGGUCGUCCUGAACAUCCUCGa -3' miRNA: 3'- -CAAGUGCCAGCAGGACUUGUAGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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