Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22588 | 5' | -50.5 | NC_005091.1 | + | 19164 | 0.67 | 0.900388 |
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Target: 5'- cGUCGAuGAAcugGUC-GCGaucaCCGUGGGCGAu -3' miRNA: 3'- -UAGUU-CUUa--UAGuUGC----GGCACCCGCU- -5' |
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| 22588 | 5' | -50.5 | NC_005091.1 | + | 45666 | 0.67 | 0.893835 |
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Target: 5'- cGUCAGGAAgc-CGACGCCGgccagcuucacaucGGCGAc -3' miRNA: 3'- -UAGUUCUUauaGUUGCGGCac------------CCGCU- -5' |
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| 22588 | 5' | -50.5 | NC_005091.1 | + | 42250 | 0.67 | 0.877651 |
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Target: 5'- cGUCgGGGAAUAaCcgUGCCG-GGGCGAa -3' miRNA: 3'- -UAG-UUCUUAUaGuuGCGGCaCCCGCU- -5' |
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| 22588 | 5' | -50.5 | NC_005091.1 | + | 14517 | 0.68 | 0.852453 |
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Target: 5'- -gUAAGcGUGccgcUCGAUGCCG-GGGCGAc -3' miRNA: 3'- uaGUUCuUAU----AGUUGCGGCaCCCGCU- -5' |
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| 22588 | 5' | -50.5 | NC_005091.1 | + | 10656 | 0.69 | 0.815411 |
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Target: 5'- cUCGGGA---UCGGC-UCGUGGGCGAc -3' miRNA: 3'- uAGUUCUuauAGUUGcGGCACCCGCU- -5' |
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| 22588 | 5' | -50.5 | NC_005091.1 | + | 20018 | 0.7 | 0.732125 |
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Target: 5'- -cCAcGAAcggcguUAUCAcUGCCGUGGGCGGc -3' miRNA: 3'- uaGUuCUU------AUAGUuGCGGCACCCGCU- -5' |
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| 22588 | 5' | -50.5 | NC_005091.1 | + | 25344 | 1.07 | 0.003903 |
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Target: 5'- cAUCAAGAAUAUCAACGCCGUGGGCGAc -3' miRNA: 3'- -UAGUUCUUAUAGUUGCGGCACCCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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