Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2259 | 3' | -63 | NC_001405.1 | + | 26156 | 0.66 | 0.260416 |
Target: 5'- cCCCCGuCCCUUUCGUAGcagaaacucuuGGcGGGCUUUg -3' miRNA: 3'- aGGGGC-GGGAGGGCAUC-----------UC-CUCGGAG- -5' |
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2259 | 3' | -63 | NC_001405.1 | + | 8671 | 0.68 | 0.176387 |
Target: 5'- cCCCUGCCCccucUCCCGgcGGGucccGAGCCc- -3' miRNA: 3'- aGGGGCGGG----AGGGCauCUC----CUCGGag -5' |
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2259 | 3' | -63 | NC_001405.1 | + | 8970 | 0.7 | 0.127458 |
Target: 5'- aCCUCGCCgccaCCGUGGAGcGAGCCg- -3' miRNA: 3'- aGGGGCGGgag-GGCAUCUC-CUCGGag -5' |
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2259 | 3' | -63 | NC_001405.1 | + | 18305 | 1.08 | 0.000114 |
Target: 5'- aUCCCCGCCCUCCCGUAGAGGAGCCUCc -3' miRNA: 3'- -AGGGGCGGGAGGGCAUCUCCUCGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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