Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22591 | 3' | -61.3 | NC_005091.1 | + | 19551 | 0.66 | 0.416054 |
Target: 5'- aGGGCacGCCGC--AGGCCGucGUguucgGCg -3' miRNA: 3'- gCCCGa-CGGCGagUCCGGCuuCGa----CG- -5' |
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22591 | 3' | -61.3 | NC_005091.1 | + | 29620 | 0.66 | 0.407052 |
Target: 5'- gCGGGCgaGCgGga-GGGCCGuacGCUGCg -3' miRNA: 3'- -GCCCGa-CGgCgagUCCGGCuu-CGACG- -5' |
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22591 | 3' | -61.3 | NC_005091.1 | + | 49893 | 0.66 | 0.38942 |
Target: 5'- gCGGuGCggUGCCGauaugCAGGCCGAcgguAGC-GCg -3' miRNA: 3'- -GCC-CG--ACGGCga---GUCCGGCU----UCGaCG- -5' |
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22591 | 3' | -61.3 | NC_005091.1 | + | 31690 | 0.67 | 0.347587 |
Target: 5'- cCGGGUgcgauaccguaGCCGCgccGGCCGccuucGGCUGCg -3' miRNA: 3'- -GCCCGa----------CGGCGaguCCGGCu----UCGACG- -5' |
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22591 | 3' | -61.3 | NC_005091.1 | + | 22197 | 0.67 | 0.339615 |
Target: 5'- -uGGCUGCCGUccCAugcGGCCGAuaugcAGCUcGCg -3' miRNA: 3'- gcCCGACGGCGa-GU---CCGGCU-----UCGA-CG- -5' |
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22591 | 3' | -61.3 | NC_005091.1 | + | 16119 | 0.67 | 0.339615 |
Target: 5'- uGGGUugUGCCGC-C-GGCCGGcGGCaGCa -3' miRNA: 3'- gCCCG--ACGGCGaGuCCGGCU-UCGaCG- -5' |
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22591 | 3' | -61.3 | NC_005091.1 | + | 15528 | 0.67 | 0.339615 |
Target: 5'- aCGGGCcGCCGCUCAGauugucagucaCCGcAAuCUGCa -3' miRNA: 3'- -GCCCGaCGGCGAGUCc----------GGC-UUcGACG- -5' |
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22591 | 3' | -61.3 | NC_005091.1 | + | 4343 | 0.69 | 0.280657 |
Target: 5'- uGGGCgUGcCCGCUCGaccuGCCuaccAAGCUGCa -3' miRNA: 3'- gCCCG-AC-GGCGAGUc---CGGc---UUCGACG- -5' |
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22591 | 3' | -61.3 | NC_005091.1 | + | 16624 | 0.69 | 0.278612 |
Target: 5'- gGGGCUGgacuccuguugcgaCCgGCUCGGGuauccCCGAAGCgccgGCa -3' miRNA: 3'- gCCCGAC--------------GG-CGAGUCC-----GGCUUCGa---CG- -5' |
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22591 | 3' | -61.3 | NC_005091.1 | + | 25214 | 0.69 | 0.273887 |
Target: 5'- gGGGCgGCCGCUgCaccgGGGUCGAugcAGCcgGCg -3' miRNA: 3'- gCCCGaCGGCGA-G----UCCGGCU---UCGa-CG- -5' |
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22591 | 3' | -61.3 | NC_005091.1 | + | 14514 | 0.69 | 0.248112 |
Target: 5'- cCGGuaaGCgUGCCGCUCGauGCCGggGCgacgGCu -3' miRNA: 3'- -GCC---CG-ACGGCGAGUc-CGGCuuCGa---CG- -5' |
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22591 | 3' | -61.3 | NC_005091.1 | + | 6622 | 0.7 | 0.235994 |
Target: 5'- cCGGGCUGCCggcGUUCAuGCCcGAGC-GCg -3' miRNA: 3'- -GCCCGACGG---CGAGUcCGGcUUCGaCG- -5' |
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22591 | 3' | -61.3 | NC_005091.1 | + | 11400 | 0.7 | 0.230124 |
Target: 5'- aCGcGGCaGCCGCUCaacaAGGCguUGAAGCcGCg -3' miRNA: 3'- -GC-CCGaCGGCGAG----UCCG--GCUUCGaCG- -5' |
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22591 | 3' | -61.3 | NC_005091.1 | + | 12967 | 0.7 | 0.230124 |
Target: 5'- uGGGCcacgGUCGUcCGGGCCG-AGCgGCg -3' miRNA: 3'- gCCCGa---CGGCGaGUCCGGCuUCGaCG- -5' |
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22591 | 3' | -61.3 | NC_005091.1 | + | 2800 | 0.7 | 0.226661 |
Target: 5'- uCGGGCUGCCGUucgcgagagauaucgUcCGGGUcaucggCGAAGCUGa -3' miRNA: 3'- -GCCCGACGGCG---------------A-GUCCG------GCUUCGACg -5' |
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22591 | 3' | -61.3 | NC_005091.1 | + | 19538 | 0.7 | 0.224377 |
Target: 5'- -cGGUUGCUGCgcuuUCGGGCCGAGcGCcGCc -3' miRNA: 3'- gcCCGACGGCG----AGUCCGGCUU-CGaCG- -5' |
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22591 | 3' | -61.3 | NC_005091.1 | + | 10966 | 0.7 | 0.224377 |
Target: 5'- -aGGCcgGCCGC-CGGGUCG-AGCUGUu -3' miRNA: 3'- gcCCGa-CGGCGaGUCCGGCuUCGACG- -5' |
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22591 | 3' | -61.3 | NC_005091.1 | + | 44493 | 0.7 | 0.210004 |
Target: 5'- cCGGGCUucuucGCUGCacgucgagacgauauUaCAGGCCGucGCUGCc -3' miRNA: 3'- -GCCCGA-----CGGCG---------------A-GUCCGGCuuCGACG- -5' |
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22591 | 3' | -61.3 | NC_005091.1 | + | 718 | 0.72 | 0.156048 |
Target: 5'- cCGGGCUGCUGCgUgAGGUCG-AGC-GCc -3' miRNA: 3'- -GCCCGACGGCG-AgUCCGGCuUCGaCG- -5' |
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22591 | 3' | -61.3 | NC_005091.1 | + | 26422 | 0.76 | 0.085303 |
Target: 5'- gGGGCUGCCaucguagcggccggGCgcgagCAGGCCGccagagAGGCUGCu -3' miRNA: 3'- gCCCGACGG--------------CGa----GUCCGGC------UUCGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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