Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22594 | 3' | -62.4 | NC_005091.1 | + | 10051 | 0.66 | 0.386255 |
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Target: 5'- -gCCGGGCGCugggagcaggaGUGGGGCUucaucacgaACCCCaacuUCa -3' miRNA: 3'- ugGGCCUGCG-----------CACCCUGA---------UGGGGg---AG- -5' |
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| 22594 | 3' | -62.4 | NC_005091.1 | + | 54901 | 0.66 | 0.369498 |
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Target: 5'- cGCCCGGACGgGUcGGAagUUACUUCCg- -3' miRNA: 3'- -UGGGCCUGCgCAcCCU--GAUGGGGGag -5' |
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| 22594 | 3' | -62.4 | NC_005091.1 | + | 16595 | 0.67 | 0.34531 |
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Target: 5'- cGCUCGG-UGCGUcGGGAaUGCCuCCCUg -3' miRNA: 3'- -UGGGCCuGCGCA-CCCUgAUGG-GGGAg -5' |
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| 22594 | 3' | -62.4 | NC_005091.1 | + | 14806 | 0.67 | 0.337504 |
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Target: 5'- uACCCGGAU-CG-GGGACgagACCCgCCg- -3' miRNA: 3'- -UGGGCCUGcGCaCCCUGa--UGGG-GGag -5' |
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| 22594 | 3' | -62.4 | NC_005091.1 | + | 14926 | 0.67 | 0.337504 |
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Target: 5'- uACCCGGAU-CG-GGGACgagACCCgCCg- -3' miRNA: 3'- -UGGGCCUGcGCaCCCUGa--UGGG-GGag -5' |
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| 22594 | 3' | -62.4 | NC_005091.1 | + | 15045 | 0.67 | 0.337504 |
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Target: 5'- uACCCGGAU-CG-GGGACgagACCCgCCg- -3' miRNA: 3'- -UGGGCCUGcGCaCCCUGa--UGGG-GGag -5' |
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| 22594 | 3' | -62.4 | NC_005091.1 | + | 27289 | 1.09 | 0.00025 |
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Target: 5'- cACCCGGACGCGUGGGACUACCCCCUCg -3' miRNA: 3'- -UGGGCCUGCGCACCCUGAUGGGGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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