Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
226 | 3' | -53.4 | AC_000008.1 | + | 32783 | 0.66 | 0.669644 |
Target: 5'- -aGCAGGUGaCAGUu-GCCGUccgAGCa -3' miRNA: 3'- ugCGUCCAC-GUCGucCGGUAuaaUCG- -5' |
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226 | 3' | -53.4 | AC_000008.1 | + | 9911 | 0.66 | 0.668479 |
Target: 5'- gACGCAGGcgGUaagcuccgcauuuGGCGGGCgGgAUUGGUc -3' miRNA: 3'- -UGCGUCCa-CG-------------UCGUCCGgUaUAAUCG- -5' |
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226 | 3' | -53.4 | AC_000008.1 | + | 12160 | 0.66 | 0.65798 |
Target: 5'- cGCGCccuGGagGCAGCugGGGCCGgaccugGGCu -3' miRNA: 3'- -UGCGu--CCa-CGUCG--UCCGGUauaa--UCG- -5' |
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226 | 3' | -53.4 | AC_000008.1 | + | 32083 | 0.66 | 0.65798 |
Target: 5'- gGCGguGGcUGCAGC-GGCUgaAgcGGCg -3' miRNA: 3'- -UGCguCC-ACGUCGuCCGGuaUaaUCG- -5' |
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226 | 3' | -53.4 | AC_000008.1 | + | 21973 | 0.66 | 0.65798 |
Target: 5'- gACGaCGGGUG-GGCGGGCgCGggccUGGCa -3' miRNA: 3'- -UGC-GUCCACgUCGUCCG-GUaua-AUCG- -5' |
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226 | 3' | -53.4 | AC_000008.1 | + | 12680 | 0.66 | 0.634581 |
Target: 5'- gGCGCAGcGUgagcgcgcgcaGCAGCAGGgCAaccugGGCu -3' miRNA: 3'- -UGCGUC-CA-----------CGUCGUCCgGUauaa-UCG- -5' |
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226 | 3' | -53.4 | AC_000008.1 | + | 10003 | 0.66 | 0.622872 |
Target: 5'- cUGCGGcG-GCGGCGgaguuuGGCCGUAggUGGCg -3' miRNA: 3'- uGCGUC-CaCGUCGU------CCGGUAUa-AUCG- -5' |
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226 | 3' | -53.4 | AC_000008.1 | + | 19460 | 0.67 | 0.599493 |
Target: 5'- -gGCGGGUGCGcacgggcacgcGCAGGCCGcuaaccgAGUc -3' miRNA: 3'- ugCGUCCACGU-----------CGUCCGGUauaa---UCG- -5' |
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226 | 3' | -53.4 | AC_000008.1 | + | 9488 | 0.67 | 0.598327 |
Target: 5'- gGCGCAuggucucGGUGaCGGCGcGGCCGUucucgcgggGGCg -3' miRNA: 3'- -UGCGU-------CCAC-GUCGU-CCGGUAuaa------UCG- -5' |
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226 | 3' | -53.4 | AC_000008.1 | + | 9741 | 0.67 | 0.587845 |
Target: 5'- -gGCGGGcgGCAGCGGGCgGcggucgggguUGUuucUGGCg -3' miRNA: 3'- ugCGUCCa-CGUCGUCCGgU----------AUA---AUCG- -5' |
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226 | 3' | -53.4 | AC_000008.1 | + | 11755 | 0.67 | 0.580876 |
Target: 5'- aGCGgGGGUGCcucgacggggaagguGGUAGGCgc-GUUGGCg -3' miRNA: 3'- -UGCgUCCACG---------------UCGUCCGguaUAAUCG- -5' |
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226 | 3' | -53.4 | AC_000008.1 | + | 17433 | 0.67 | 0.576239 |
Target: 5'- -gGguGGUG-GGCAGGCCuugcuUUAGUg -3' miRNA: 3'- ugCguCCACgUCGUCCGGuau--AAUCG- -5' |
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226 | 3' | -53.4 | AC_000008.1 | + | 15056 | 0.67 | 0.564686 |
Target: 5'- gGCGCAGGcgGCAGCAacaGCaGUGgcAGCg -3' miRNA: 3'- -UGCGUCCa-CGUCGUc--CGgUAUaaUCG- -5' |
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226 | 3' | -53.4 | AC_000008.1 | + | 8929 | 0.67 | 0.561231 |
Target: 5'- cACGguGGcgGCGaggucguuggaaauGCGGGCCAUG--AGCu -3' miRNA: 3'- -UGCguCCa-CGU--------------CGUCCGGUAUaaUCG- -5' |
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226 | 3' | -53.4 | AC_000008.1 | + | 13270 | 0.67 | 0.553194 |
Target: 5'- cGCGCAGG-GCGG-AGGCCccaacuGCg -3' miRNA: 3'- -UGCGUCCaCGUCgUCCGGuauaauCG- -5' |
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226 | 3' | -53.4 | AC_000008.1 | + | 22677 | 0.67 | 0.553194 |
Target: 5'- aGCGcCGGGUGguGCAcgcuGGCCAgcac-GCu -3' miRNA: 3'- -UGC-GUCCACguCGU----CCGGUauaauCG- -5' |
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226 | 3' | -53.4 | AC_000008.1 | + | 20110 | 0.68 | 0.530431 |
Target: 5'- -gGCAGGUGCGGCGucuGGCgucaGUAaUGGUc -3' miRNA: 3'- ugCGUCCACGUCGU---CCGg---UAUaAUCG- -5' |
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226 | 3' | -53.4 | AC_000008.1 | + | 9200 | 0.68 | 0.530431 |
Target: 5'- cCGCcGGUGCAGUauGGGCUGUAgagaugacggUAGUa -3' miRNA: 3'- uGCGuCCACGUCG--UCCGGUAUa---------AUCG- -5' |
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226 | 3' | -53.4 | AC_000008.1 | + | 8521 | 0.68 | 0.508019 |
Target: 5'- cAC-CAGG-GCAGCGGGCCAa------ -3' miRNA: 3'- -UGcGUCCaCGUCGUCCGGUauaaucg -5' |
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226 | 3' | -53.4 | AC_000008.1 | + | 18219 | 0.68 | 0.496963 |
Target: 5'- aACGgAGGcgGCAGgugaGGGCCAUAUcuGCa -3' miRNA: 3'- -UGCgUCCa-CGUCg---UCCGGUAUAauCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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