Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 226 | 3' | -53.4 | AC_000008.1 | + | 12680 | 0.66 | 0.634581 |
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Target: 5'- gGCGCAGcGUgagcgcgcgcaGCAGCAGGgCAaccugGGCu -3' miRNA: 3'- -UGCGUC-CA-----------CGUCGUCCgGUauaa-UCG- -5' |
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| 226 | 3' | -53.4 | AC_000008.1 | + | 21973 | 0.66 | 0.65798 |
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Target: 5'- gACGaCGGGUG-GGCGGGCgCGggccUGGCa -3' miRNA: 3'- -UGC-GUCCACgUCGUCCG-GUaua-AUCG- -5' |
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| 226 | 3' | -53.4 | AC_000008.1 | + | 12160 | 0.66 | 0.65798 |
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Target: 5'- cGCGCccuGGagGCAGCugGGGCCGgaccugGGCu -3' miRNA: 3'- -UGCGu--CCa-CGUCG--UCCGGUauaa--UCG- -5' |
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| 226 | 3' | -53.4 | AC_000008.1 | + | 32083 | 0.66 | 0.65798 |
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Target: 5'- gGCGguGGcUGCAGC-GGCUgaAgcGGCg -3' miRNA: 3'- -UGCguCC-ACGUCGuCCGGuaUaaUCG- -5' |
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| 226 | 3' | -53.4 | AC_000008.1 | + | 9911 | 0.66 | 0.668479 |
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Target: 5'- gACGCAGGcgGUaagcuccgcauuuGGCGGGCgGgAUUGGUc -3' miRNA: 3'- -UGCGUCCa-CG-------------UCGUCCGgUaUAAUCG- -5' |
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| 226 | 3' | -53.4 | AC_000008.1 | + | 32783 | 0.66 | 0.669644 |
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Target: 5'- -aGCAGGUGaCAGUu-GCCGUccgAGCa -3' miRNA: 3'- ugCGUCCAC-GUCGucCGGUAuaaUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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