Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
226 | 5' | -59.2 | AC_000008.1 | + | 23742 | 0.66 | 0.39868 |
Target: 5'- gCGUCGGCUUggGGCCCaGCgcaggUCAGCg- -3' miRNA: 3'- aGCGGCUGGA--UCGGGaCGa----AGUCGgc -5' |
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226 | 5' | -59.2 | AC_000008.1 | + | 1831 | 0.66 | 0.36632 |
Target: 5'- aCGcCCGcACCUGGUuuUGCUUCAGgaaauaugacuacguCCGg -3' miRNA: 3'- aGC-GGC-UGGAUCGggACGAAGUC---------------GGC- -5' |
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226 | 5' | -59.2 | AC_000008.1 | + | 20674 | 0.67 | 0.345774 |
Target: 5'- -gGgCGGCagcuucGGCCgCUGCUUCGGCCu -3' miRNA: 3'- agCgGCUGga----UCGG-GACGAAGUCGGc -5' |
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226 | 5' | -59.2 | AC_000008.1 | + | 33713 | 0.67 | 0.321273 |
Target: 5'- cCGCCGccGCCUGGCuuccuCCUGCUgCuGCUGc -3' miRNA: 3'- aGCGGC--UGGAUCG-----GGACGAaGuCGGC- -5' |
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226 | 5' | -59.2 | AC_000008.1 | + | 17524 | 0.68 | 0.283375 |
Target: 5'- gUCGCCGucGCC-AGCCCgUGCU--GGCCc -3' miRNA: 3'- -AGCGGC--UGGaUCGGG-ACGAagUCGGc -5' |
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226 | 5' | -59.2 | AC_000008.1 | + | 26120 | 0.68 | 0.262383 |
Target: 5'- cCGCCG-CCgcAGCCCUaucaGCagCAGCCGc -3' miRNA: 3'- aGCGGCuGGa-UCGGGA----CGaaGUCGGC- -5' |
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226 | 5' | -59.2 | AC_000008.1 | + | 3768 | 0.69 | 0.230229 |
Target: 5'- aCGCCGuuggagacugcaGCCUccGCCgCcGCUUCAGCCGc -3' miRNA: 3'- aGCGGC------------UGGAu-CGG-GaCGAAGUCGGC- -5' |
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226 | 5' | -59.2 | AC_000008.1 | + | 5408 | 0.75 | 0.092035 |
Target: 5'- cUGCCGGuCUUcGCCCUGCgcgUCGGCCa -3' miRNA: 3'- aGCGGCU-GGAuCGGGACGa--AGUCGGc -5' |
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226 | 5' | -59.2 | AC_000008.1 | + | 12575 | 0.77 | 0.068887 |
Target: 5'- -gGCCGGCCUGGUCuacgacgcgCUGCUUCAGCgCGu -3' miRNA: 3'- agCGGCUGGAUCGG---------GACGAAGUCG-GC- -5' |
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226 | 5' | -59.2 | AC_000008.1 | + | 23322 | 0.77 | 0.059348 |
Target: 5'- -gGCCGGCCUcgucgggccggauGGCCCUGuUUUCGGCCa -3' miRNA: 3'- agCGGCUGGA-------------UCGGGAC-GAAGUCGGc -5' |
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226 | 5' | -59.2 | AC_000008.1 | + | 25802 | 1.09 | 0.000209 |
Target: 5'- gUCGCCGACCUAGCCCUGCUUCAGCCGa -3' miRNA: 3'- -AGCGGCUGGAUCGGGACGAAGUCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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