Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22603 | 3' | -56.9 | NC_005091.1 | + | 56049 | 0.66 | 0.651959 |
Target: 5'- uGUCGAGCGUCuUGCGC-AGCaa-ACGGc -3' miRNA: 3'- -UAGCUCGUAG-ACGUGcUCGcggUGCC- -5' |
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22603 | 3' | -56.9 | NC_005091.1 | + | 21699 | 0.66 | 0.630172 |
Target: 5'- gGUCGGGCGUUUGCGuCGucuGCGUauaGCGc -3' miRNA: 3'- -UAGCUCGUAGACGU-GCu--CGCGg--UGCc -5' |
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22603 | 3' | -56.9 | NC_005091.1 | + | 30518 | 0.66 | 0.608392 |
Target: 5'- -gCGGcGCGcUCUGCgucAUGAGCaucGCCACGGa -3' miRNA: 3'- uaGCU-CGU-AGACG---UGCUCG---CGGUGCC- -5' |
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22603 | 3' | -56.9 | NC_005091.1 | + | 31271 | 0.67 | 0.575894 |
Target: 5'- cAUCGgcGGCAUgucgUUGCAgGAGCGCUuccaaGCGGu -3' miRNA: 3'- -UAGC--UCGUA----GACGUgCUCGCGG-----UGCC- -5' |
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22603 | 3' | -56.9 | NC_005091.1 | + | 718 | 0.67 | 0.575894 |
Target: 5'- -cCGGGCugCUGCGugagguCGAGCGCCAgUGGa -3' miRNA: 3'- uaGCUCGuaGACGU------GCUCGCGGU-GCC- -5' |
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22603 | 3' | -56.9 | NC_005091.1 | + | 31785 | 0.67 | 0.565141 |
Target: 5'- -aCGAGCAggCcGCcgACGAGCGCgACGa -3' miRNA: 3'- uaGCUCGUa-GaCG--UGCUCGCGgUGCc -5' |
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22603 | 3' | -56.9 | NC_005091.1 | + | 19873 | 0.68 | 0.53323 |
Target: 5'- cGUCGGcaaucGCAUCacgcuCAUGGGCGCUGCGGa -3' miRNA: 3'- -UAGCU-----CGUAGac---GUGCUCGCGGUGCC- -5' |
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22603 | 3' | -56.9 | NC_005091.1 | + | 45284 | 0.68 | 0.522733 |
Target: 5'- gAUCGAGaaggcgGUCggGCACGAGUauuuGCCGCGa -3' miRNA: 3'- -UAGCUCg-----UAGa-CGUGCUCG----CGGUGCc -5' |
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22603 | 3' | -56.9 | NC_005091.1 | + | 40 | 0.68 | 0.516473 |
Target: 5'- aGUCGGGCGUUccuaucaugggGCugauuuccauucaagGCGuGCGCCACGGc -3' miRNA: 3'- -UAGCUCGUAGa----------CG---------------UGCuCGCGGUGCC- -5' |
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22603 | 3' | -56.9 | NC_005091.1 | + | 57434 | 0.68 | 0.516473 |
Target: 5'- aGUCGGGCGUUccuaucaugggGCugauuuccauucaagGCGuGCGCCACGGc -3' miRNA: 3'- -UAGCUCGUAGa----------CG---------------UGCuCGCGGUGCC- -5' |
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22603 | 3' | -56.9 | NC_005091.1 | + | 28428 | 0.68 | 0.513354 |
Target: 5'- gGUCGAGCAUCgUGCuggcgacggccaguuCGAGCGaCgGCGa -3' miRNA: 3'- -UAGCUCGUAG-ACGu--------------GCUCGC-GgUGCc -5' |
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22603 | 3' | -56.9 | NC_005091.1 | + | 51577 | 0.68 | 0.512316 |
Target: 5'- uGUCGuGCGUCgcgugacggGCGCGcagaAGCGCggCACGGg -3' miRNA: 3'- -UAGCuCGUAGa--------CGUGC----UCGCG--GUGCC- -5' |
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22603 | 3' | -56.9 | NC_005091.1 | + | 23744 | 0.68 | 0.491748 |
Target: 5'- uAUCGAGCAgcuUCUGCAUcucAGCGaCCAUGc -3' miRNA: 3'- -UAGCUCGU---AGACGUGc--UCGC-GGUGCc -5' |
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22603 | 3' | -56.9 | NC_005091.1 | + | 18129 | 0.69 | 0.461638 |
Target: 5'- gAUCGAGaacaCGUCggucgGCGCGAGCuUCGCGGc -3' miRNA: 3'- -UAGCUC----GUAGa----CGUGCUCGcGGUGCC- -5' |
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22603 | 3' | -56.9 | NC_005091.1 | + | 1408 | 0.69 | 0.461638 |
Target: 5'- -aUGGGCGgcaUGC-CGAGCGCCAaGGa -3' miRNA: 3'- uaGCUCGUag-ACGuGCUCGCGGUgCC- -5' |
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22603 | 3' | -56.9 | NC_005091.1 | + | 11325 | 0.69 | 0.455733 |
Target: 5'- uGUUGAGCggCUgccgcguucgacugcGCAUGuGCGUCACGGa -3' miRNA: 3'- -UAGCUCGuaGA---------------CGUGCuCGCGGUGCC- -5' |
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22603 | 3' | -56.9 | NC_005091.1 | + | 26143 | 0.69 | 0.43253 |
Target: 5'- uGUCGuGGUAUCaGCGCGuGCGCgGCGa -3' miRNA: 3'- -UAGC-UCGUAGaCGUGCuCGCGgUGCc -5' |
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22603 | 3' | -56.9 | NC_005091.1 | + | 9068 | 0.7 | 0.377682 |
Target: 5'- -gCGGGCuuuUCUGUACGAcGCGCuCGcCGGg -3' miRNA: 3'- uaGCUCGu--AGACGUGCU-CGCG-GU-GCC- -5' |
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22603 | 3' | -56.9 | NC_005091.1 | + | 36300 | 0.72 | 0.312177 |
Target: 5'- --aGAcGCAa-UGCGCGAGCGUCGCGGc -3' miRNA: 3'- uagCU-CGUagACGUGCUCGCGGUGCC- -5' |
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22603 | 3' | -56.9 | NC_005091.1 | + | 48714 | 0.75 | 0.197126 |
Target: 5'- -gCG-GCA-CUGCACGAGCGaCACGGa -3' miRNA: 3'- uaGCuCGUaGACGUGCUCGCgGUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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