Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22603 | 5' | -54.6 | NC_005091.1 | + | 46710 | 0.66 | 0.778282 |
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Target: 5'- uUCCUGCucACGCCACaGgacgUGUUGgaAGGcCg -3' miRNA: 3'- -AGGACGu-UGCGGUG-Ca---ACAGC--UCCaG- -5' |
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| 22603 | 5' | -54.6 | NC_005091.1 | + | 18223 | 0.66 | 0.757949 |
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Target: 5'- ---cGCAuCGCCACGcgcaucgCGAGGUCg -3' miRNA: 3'- aggaCGUuGCGGUGCaaca---GCUCCAG- -5' |
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| 22603 | 5' | -54.6 | NC_005091.1 | + | 14552 | 0.66 | 0.737099 |
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Target: 5'- cCCUGuCAcgauCGCUACGg---CGGGGUCg -3' miRNA: 3'- aGGAC-GUu---GCGGUGCaacaGCUCCAG- -5' |
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| 22603 | 5' | -54.6 | NC_005091.1 | + | 34367 | 0.67 | 0.703975 |
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Target: 5'- -aCUGC-ACGCCGCGUcgaagcgugggcgUGUCGAGu-- -3' miRNA: 3'- agGACGuUGCGGUGCA-------------ACAGCUCcag -5' |
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| 22603 | 5' | -54.6 | NC_005091.1 | + | 20116 | 0.67 | 0.683316 |
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Target: 5'- aCCgUGCccauccACGCCACGUccgcuucguUGUCcGGGUCg -3' miRNA: 3'- aGG-ACGu-----UGCGGUGCA---------ACAGcUCCAG- -5' |
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| 22603 | 5' | -54.6 | NC_005091.1 | + | 40880 | 0.68 | 0.617259 |
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Target: 5'- aCCgUGCAgaGCGCCcaguCGUUGUCGccGUCc -3' miRNA: 3'- aGG-ACGU--UGCGGu---GCAACAGCucCAG- -5' |
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| 22603 | 5' | -54.6 | NC_005091.1 | + | 57343 | 0.69 | 0.551641 |
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Target: 5'- cUCCUGcCGACGCCGgGUUG-CGGGa-- -3' miRNA: 3'- -AGGAC-GUUGCGGUgCAACaGCUCcag -5' |
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| 22603 | 5' | -54.6 | NC_005091.1 | + | 55975 | 0.7 | 0.540882 |
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Target: 5'- gCCUGCuGCGCCAuCGUgcgaccggGaUCGAuGGUCa -3' miRNA: 3'- aGGACGuUGCGGU-GCAa-------C-AGCU-CCAG- -5' |
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| 22603 | 5' | -54.6 | NC_005091.1 | + | 30311 | 1.11 | 0.00091 |
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Target: 5'- uUCCUGCAACGCCACGUUGUCGAGGUCg -3' miRNA: 3'- -AGGACGUUGCGGUGCAACAGCUCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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