Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22604 | 3' | -53.5 | NC_005091.1 | + | 7409 | 0.67 | 0.75615 |
Target: 5'- cUCGgcuUGGUCGAGCUGCccgaaGAACCg---- -3' miRNA: 3'- -GGC---ACCAGCUCGACGa----CUUGGacaag -5' |
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22604 | 3' | -53.5 | NC_005091.1 | + | 34385 | 0.71 | 0.559588 |
Target: 5'- gCGUGGgcgugUCGAGUUGCUGGuACggGUUCg -3' miRNA: 3'- gGCACC-----AGCUCGACGACU-UGgaCAAG- -5' |
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22604 | 3' | -53.5 | NC_005091.1 | + | 41204 | 0.72 | 0.485203 |
Target: 5'- cCCaUGGuUUGAGCUGCUGcggaCUGUUCg -3' miRNA: 3'- -GGcACC-AGCUCGACGACuug-GACAAG- -5' |
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22604 | 3' | -53.5 | NC_005091.1 | + | 28425 | 0.75 | 0.312281 |
Target: 5'- -aGUGGUCGAGCaucgUGCUGGcgacgGCCaGUUCg -3' miRNA: 3'- ggCACCAGCUCG----ACGACU-----UGGaCAAG- -5' |
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22604 | 3' | -53.5 | NC_005091.1 | + | 30926 | 1.11 | 0.00111 |
Target: 5'- gCCGUGGUCGAGCUGCUGAACCUGUUCu -3' miRNA: 3'- -GGCACCAGCUCGACGACUUGGACAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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