Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2261 | 3' | -57.3 | NC_001405.1 | + | 886 | 0.66 | 0.510973 |
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Target: 5'- uGCCGGGaaaggugagGCGgCUcCGGAGaACC-GGGCg -3' miRNA: 3'- -CGGUCC---------UGCgGA-GCCUCaUGGaCUCG- -5' |
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| 2261 | 3' | -57.3 | NC_001405.1 | + | 10232 | 0.66 | 0.500421 |
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Target: 5'- cCCGGcuGCGagagCUCGGuGUACCUGAGa -3' miRNA: 3'- cGGUCc-UGCg---GAGCCuCAUGGACUCg -5' |
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| 2261 | 3' | -57.3 | NC_001405.1 | + | 12195 | 0.66 | 0.500421 |
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Target: 5'- uCCAGGGCGCgUCGGcuuGGgGCCc-AGCg -3' miRNA: 3'- cGGUCCUGCGgAGCC---UCaUGGacUCG- -5' |
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| 2261 | 3' | -57.3 | NC_001405.1 | + | 17059 | 0.67 | 0.459243 |
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Target: 5'- cGCCAGcgacuggaaGAUGUCUUGGAaaaaaugaccGUggaGCCUGGGCu -3' miRNA: 3'- -CGGUC---------CUGCGGAGCCU----------CA---UGGACUCG- -5' |
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| 2261 | 3' | -57.3 | NC_001405.1 | + | 12144 | 0.68 | 0.386358 |
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Target: 5'- aGCCGacGCGCCcUGGAGgcagcuggggccggACCUGGGCu -3' miRNA: 3'- -CGGUccUGCGGaGCCUCa-------------UGGACUCG- -5' |
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| 2261 | 3' | -57.3 | NC_001405.1 | + | 16024 | 0.7 | 0.292292 |
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Target: 5'- cCCAGGGCGCg-CGGuAGUGCCcGcGCa -3' miRNA: 3'- cGGUCCUGCGgaGCC-UCAUGGaCuCG- -5' |
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| 2261 | 3' | -57.3 | NC_001405.1 | + | 14864 | 0.7 | 0.277759 |
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Target: 5'- uGCCAGGAUGCggggUGGAcUucacccacagccGCCUGAGCa -3' miRNA: 3'- -CGGUCCUGCGga--GCCUcA------------UGGACUCG- -5' |
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| 2261 | 3' | -57.3 | NC_001405.1 | + | 27513 | 0.71 | 0.25705 |
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Target: 5'- gGCCuGGGCGUCUCuGGgaAGUACCacagugguggGAGCg -3' miRNA: 3'- -CGGuCCUGCGGAG-CC--UCAUGGa---------CUCG- -5' |
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| 2261 | 3' | -57.3 | NC_001405.1 | + | 18868 | 1.12 | 0.000226 |
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Target: 5'- gGCCAGGACGCCUCGGAGUACCUGAGCc -3' miRNA: 3'- -CGGUCCUGCGGAGCCUCAUGGACUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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