Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22612 | 3' | -48.8 | NC_005091.1 | + | 26668 | 0.66 | 0.979286 |
Target: 5'- gGCGGcGGCCGGGacuUCGAaCGACUcacccagcgcgucgcGAAGGc -3' miRNA: 3'- -CGUU-CCGGUCU---AGCUaGUUGA---------------CUUCC- -5' |
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22612 | 3' | -48.8 | NC_005091.1 | + | 432 | 0.67 | 0.966003 |
Target: 5'- cGCGAcGGCgCAGGUCGAgcagCAAUcGgcGGu -3' miRNA: 3'- -CGUU-CCG-GUCUAGCUa---GUUGaCuuCC- -5' |
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22612 | 3' | -48.8 | NC_005091.1 | + | 57826 | 0.67 | 0.966003 |
Target: 5'- cGCGAcGGCgCAGGUCGAgcagCAAUcGgcGGu -3' miRNA: 3'- -CGUU-CCG-GUCUAGCUa---GUUGaCuuCC- -5' |
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22612 | 3' | -48.8 | NC_005091.1 | + | 56131 | 0.67 | 0.962297 |
Target: 5'- cGCAAGGCUAGcGUCGAacUCgGGCUGccGa -3' miRNA: 3'- -CGUUCCGGUC-UAGCU--AG-UUGACuuCc -5' |
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22612 | 3' | -48.8 | NC_005091.1 | + | 6442 | 0.67 | 0.95832 |
Target: 5'- cCGAGGgCAGcgUGcgCGcugcGCUGAAGGa -3' miRNA: 3'- cGUUCCgGUCuaGCuaGU----UGACUUCC- -5' |
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22612 | 3' | -48.8 | NC_005091.1 | + | 31065 | 0.68 | 0.944694 |
Target: 5'- cGCAgcgugggaucAGGCCAcGUcCGAUCcGCUGggGa -3' miRNA: 3'- -CGU----------UCCGGUcUA-GCUAGuUGACuuCc -5' |
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22612 | 3' | -48.8 | NC_005091.1 | + | 25723 | 0.68 | 0.939572 |
Target: 5'- uGCGcGGCCAGcucggCGAUCGGCUGc--- -3' miRNA: 3'- -CGUuCCGGUCua---GCUAGUUGACuucc -5' |
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22612 | 3' | -48.8 | NC_005091.1 | + | 36485 | 0.68 | 0.936356 |
Target: 5'- uGCGGcGGCCGGAUCGAagcggaUCAGCgcaacgaucucgaccUGuGGGg -3' miRNA: 3'- -CGUU-CCGGUCUAGCU------AGUUG---------------ACuUCC- -5' |
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22612 | 3' | -48.8 | NC_005091.1 | + | 48898 | 0.68 | 0.928438 |
Target: 5'- uGCGAuGGCCGGGUC---UAGCUGGgcGGGa -3' miRNA: 3'- -CGUU-CCGGUCUAGcuaGUUGACU--UCC- -5' |
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22612 | 3' | -48.8 | NC_005091.1 | + | 38168 | 0.68 | 0.922424 |
Target: 5'- uGCcGGGCgAGAcgacCGAUgAGCUGAAGa -3' miRNA: 3'- -CGuUCCGgUCUa---GCUAgUUGACUUCc -5' |
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22612 | 3' | -48.8 | NC_005091.1 | + | 34957 | 0.69 | 0.916113 |
Target: 5'- uCAuGGUCAGAa-GGUgAACUGAAGGg -3' miRNA: 3'- cGUuCCGGUCUagCUAgUUGACUUCC- -5' |
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22612 | 3' | -48.8 | NC_005091.1 | + | 33704 | 0.69 | 0.902602 |
Target: 5'- cGCcGGGCCAGAcgCGGgaccuguucuaUCAGCcgcugaUGAAGGa -3' miRNA: 3'- -CGuUCCGGUCUa-GCU-----------AGUUG------ACUUCC- -5' |
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22612 | 3' | -48.8 | NC_005091.1 | + | 1247 | 0.71 | 0.837378 |
Target: 5'- gGCuGGGCCGGAugUCGGaaGGCUGggGc -3' miRNA: 3'- -CGuUCCGGUCU--AGCUagUUGACuuCc -5' |
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22612 | 3' | -48.8 | NC_005091.1 | + | 29825 | 0.73 | 0.701482 |
Target: 5'- aCGAGGaCCAGAaugagcaUCGAaaUCGucGCUGAAGGg -3' miRNA: 3'- cGUUCC-GGUCU-------AGCU--AGU--UGACUUCC- -5' |
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22612 | 3' | -48.8 | NC_005091.1 | + | 28952 | 0.77 | 0.489284 |
Target: 5'- uGCAGGGCUGGGUCaaccauGCUGAAGGg -3' miRNA: 3'- -CGUUCCGGUCUAGcuagu-UGACUUCC- -5' |
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22612 | 3' | -48.8 | NC_005091.1 | + | 33626 | 1.15 | 0.002139 |
Target: 5'- cGCAAGGCCAGAUCGAUCAACUGAAGGa -3' miRNA: 3'- -CGUUCCGGUCUAGCUAGUUGACUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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