Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22617 | 5' | -54.8 | NC_005091.1 | + | 48721 | 0.66 | 0.738586 |
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Target: 5'- cAGGcuacGCGGcgGG-CUGCUGCGUGuUGGu -3' miRNA: 3'- uUCC----UGUUuaCCaGACGACGCGC-GCC- -5' |
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| 22617 | 5' | -54.8 | NC_005091.1 | + | 33308 | 0.67 | 0.684425 |
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Target: 5'- -cGGGCAGAUGGUC-GCcGCGCa--- -3' miRNA: 3'- uuCCUGUUUACCAGaCGaCGCGcgcc -5' |
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| 22617 | 5' | -54.8 | NC_005091.1 | + | 26388 | 0.68 | 0.595656 |
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Target: 5'- -cGGGCAuucaggcGGUCUGCUGCG-GCa- -3' miRNA: 3'- uuCCUGUuua----CCAGACGACGCgCGcc -5' |
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| 22617 | 5' | -54.8 | NC_005091.1 | + | 40597 | 0.68 | 0.5846 |
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Target: 5'- uGAGGGCAAGcgaaccGGUCUGUgggGCuaCGCGGa -3' miRNA: 3'- -UUCCUGUUUa-----CCAGACGa--CGc-GCGCC- -5' |
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| 22617 | 5' | -54.8 | NC_005091.1 | + | 56094 | 0.69 | 0.562617 |
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Target: 5'- -cGGGCGGGaGGUCgGCcgGCGCGCa- -3' miRNA: 3'- uuCCUGUUUaCCAGaCGa-CGCGCGcc -5' |
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| 22617 | 5' | -54.8 | NC_005091.1 | + | 35701 | 1.09 | 0.001098 |
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Target: 5'- cAAGGACAAAUGGUCUGCUGCGCGCGGa -3' miRNA: 3'- -UUCCUGUUUACCAGACGACGCGCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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