Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2262 | 3' | -51.9 | NC_001405.1 | + | 12245 | 0.68 | 0.700279 |
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Target: 5'- uGCCaGCGCGCG-CGgguGCCACc-GCCa -3' miRNA: 3'- -UGGaUGCGUGCuGCau-UGGUGucUGG- -5' |
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| 2262 | 3' | -51.9 | NC_001405.1 | + | 15865 | 0.68 | 0.711553 |
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Target: 5'- ---gACGCGCGGCGaGACUAUgcccaGGGCCu -3' miRNA: 3'- uggaUGCGUGCUGCaUUGGUG-----UCUGG- -5' |
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| 2262 | 3' | -51.9 | NC_001405.1 | + | 21730 | 0.67 | 0.733831 |
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Target: 5'- gGCgUGCGCAgguacacggucuCGAUGacGCCGCGGugCg -3' miRNA: 3'- -UGgAUGCGU------------GCUGCauUGGUGUCugG- -5' |
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| 2262 | 3' | -51.9 | NC_001405.1 | + | 12727 | 0.67 | 0.744809 |
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Target: 5'- -gCUGCGCGCGcucACGcugcGCCACGG-CCu -3' miRNA: 3'- ugGAUGCGUGC---UGCau--UGGUGUCuGG- -5' |
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| 2262 | 3' | -51.9 | NC_001405.1 | + | 22366 | 0.67 | 0.755662 |
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Target: 5'- aAUCUGCGCAC--UGUGGCUGCGGAa- -3' miRNA: 3'- -UGGAUGCGUGcuGCAUUGGUGUCUgg -5' |
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| 2262 | 3' | -51.9 | NC_001405.1 | + | 33518 | 0.67 | 0.766377 |
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Target: 5'- uACCUGCGCuuGugGUAugaugGCCACGuGGg- -3' miRNA: 3'- -UGGAUGCGugCugCAU-----UGGUGU-CUgg -5' |
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| 2262 | 3' | -51.9 | NC_001405.1 | + | 7873 | 0.67 | 0.766377 |
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Target: 5'- gACCgccGCGCGaGACcUAGCCGUGGACCu -3' miRNA: 3'- -UGGa--UGCGUgCUGcAUUGGUGUCUGG- -5' |
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| 2262 | 3' | -51.9 | NC_001405.1 | + | 5383 | 0.66 | 0.797561 |
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Target: 5'- aGCCcggaGCGCACcccuucacCGUGACC-CAGACUa -3' miRNA: 3'- -UGGa---UGCGUGcu------GCAUUGGuGUCUGG- -5' |
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| 2262 | 3' | -51.9 | NC_001405.1 | + | 11202 | 0.66 | 0.807594 |
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Target: 5'- cAUCUGC-CGCcGCGUcAGCCGCGGAUg -3' miRNA: 3'- -UGGAUGcGUGcUGCA-UUGGUGUCUGg -5' |
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| 2262 | 3' | -51.9 | NC_001405.1 | + | 6502 | 0.78 | 0.210481 |
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Target: 5'- cGCCUACGCGgaGAgGUAGCCACcagcguuGACCu -3' miRNA: 3'- -UGGAUGCGUg-CUgCAUUGGUGu------CUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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