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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2262 | 5' | -55.6 | NC_001405.1 | + | 13942 | 0.66 | 0.548142 |
Target: 5'- -gGGCGCggGcCGGgcaCAUCCCUGUGc- -3' miRNA: 3'- aaCUGCGa-C-GCCaa-GUAGGGACACcu -5' |
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2262 | 5' | -55.6 | NC_001405.1 | + | 8699 | 0.68 | 0.430728 |
Target: 5'- -cGACGCgGCGGUUgAUCuCCUGaaucUGGc -3' miRNA: 3'- aaCUGCGaCGCCAAgUAG-GGAC----ACCu -5' |
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2262 | 5' | -55.6 | NC_001405.1 | + | 19007 | 1.06 | 0.000704 |
Target: 5'- uUUGACGCUGCGGUUCAUCCCUGUGGAc -3' miRNA: 3'- -AACUGCGACGCCAAGUAGGGACACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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