Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22620 | 3' | -53.6 | NC_005091.1 | + | 56045 | 0.66 | 0.857707 |
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Target: 5'- uCCGgacc-UUCCUGAGcaagaugccgGUCGGCGCGu -3' miRNA: 3'- -GGCaguaaGAGGACUC----------UAGCCGCGCu -5' |
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| 22620 | 3' | -53.6 | NC_005091.1 | + | 23939 | 0.66 | 0.823771 |
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Target: 5'- gCCGUCAcgauguacaacaacCUCgUGcAGcUCGGCGCGAu -3' miRNA: 3'- -GGCAGUaa------------GAGgAC-UCuAGCCGCGCU- -5' |
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| 22620 | 3' | -53.6 | NC_005091.1 | + | 14349 | 0.66 | 0.813626 |
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Target: 5'- aCGUCAgucCUCCggucaacGGGUCGGCGUa- -3' miRNA: 3'- gGCAGUaa-GAGGac-----UCUAGCCGCGcu -5' |
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| 22620 | 3' | -53.6 | NC_005091.1 | + | 13986 | 0.68 | 0.744302 |
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Target: 5'- aCCGUCGUcgcaccgcccgUUCCgGAGAUCGcGCGCc- -3' miRNA: 3'- -GGCAGUAa----------GAGGaCUCUAGC-CGCGcu -5' |
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| 22620 | 3' | -53.6 | NC_005091.1 | + | 22075 | 0.68 | 0.744302 |
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Target: 5'- aUCGUCGUaUCaCCUGAcugGAUCGGUuucGCGAg -3' miRNA: 3'- -GGCAGUA-AGaGGACU---CUAGCCG---CGCU- -5' |
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| 22620 | 3' | -53.6 | NC_005091.1 | + | 7257 | 0.68 | 0.744302 |
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Target: 5'- uCCGUCuugCUCgUG---UCGGCGCGGg -3' miRNA: 3'- -GGCAGuaaGAGgACucuAGCCGCGCU- -5' |
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| 22620 | 3' | -53.6 | NC_005091.1 | + | 3888 | 0.7 | 0.592882 |
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Target: 5'- gCCGUaacuUUUuggcuuccgaaaUCgaGAGAUCGGCGCGAg -3' miRNA: 3'- -GGCAgu--AAG------------AGgaCUCUAGCCGCGCU- -5' |
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| 22620 | 3' | -53.6 | NC_005091.1 | + | 45429 | 0.71 | 0.580914 |
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Target: 5'- cUCGUUAcUCUCCUGcGGUCGGCacuucgaagauauGCGGg -3' miRNA: 3'- -GGCAGUaAGAGGACuCUAGCCG-------------CGCU- -5' |
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| 22620 | 3' | -53.6 | NC_005091.1 | + | 36983 | 1.11 | 0.001279 |
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Target: 5'- aCCGUCAUUCUCCUGAGAUCGGCGCGAu -3' miRNA: 3'- -GGCAGUAAGAGGACUCUAGCCGCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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