Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22621 | 5' | -52.7 | NC_005091.1 | + | 20058 | 0.66 | 0.869488 |
Target: 5'- cGACA---UCU---GCUGCCCGACGAu -3' miRNA: 3'- cCUGUacuAGGacaUGACGGGCUGCU- -5' |
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22621 | 5' | -52.7 | NC_005091.1 | + | 56797 | 0.67 | 0.835533 |
Target: 5'- -cAUGUGAacaUCUUucgGCUGCCCGGCGAu -3' miRNA: 3'- ccUGUACU---AGGAca-UGACGGGCUGCU- -5' |
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22621 | 5' | -52.7 | NC_005091.1 | + | 31535 | 0.68 | 0.76817 |
Target: 5'- uGGCGUGAUUCaGUcCgGCCCGGCGc -3' miRNA: 3'- cCUGUACUAGGaCAuGaCGGGCUGCu -5' |
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22621 | 5' | -52.7 | NC_005091.1 | + | 26643 | 0.68 | 0.757888 |
Target: 5'- cGGGCGgucGAUCgaGUcgGCgGCCCGGCGu -3' miRNA: 3'- -CCUGUa--CUAGgaCA--UGaCGGGCUGCu -5' |
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22621 | 5' | -52.7 | NC_005091.1 | + | 4850 | 0.69 | 0.704758 |
Target: 5'- cGGGCGaag-CCguaUGUGCUGCCgGACGGg -3' miRNA: 3'- -CCUGUacuaGG---ACAUGACGGgCUGCU- -5' |
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22621 | 5' | -52.7 | NC_005091.1 | + | 39284 | 0.72 | 0.539893 |
Target: 5'- gGGACAUGuacgCCgUGUGCUGCCagaGCGGa -3' miRNA: 3'- -CCUGUACua--GG-ACAUGACGGgc-UGCU- -5' |
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22621 | 5' | -52.7 | NC_005091.1 | + | 4193 | 0.74 | 0.427226 |
Target: 5'- cGGAUGUccaaGGUCCUGUcguUGCCCGGCGGc -3' miRNA: 3'- -CCUGUA----CUAGGACAug-ACGGGCUGCU- -5' |
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22621 | 5' | -52.7 | NC_005091.1 | + | 37055 | 1.12 | 0.001427 |
Target: 5'- uGGACAUGAUCCUGUACUGCCCGACGAa -3' miRNA: 3'- -CCUGUACUAGGACAUGACGGGCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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