Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22622 | 3' | -61.2 | NC_005091.1 | + | 31754 | 0.66 | 0.415153 |
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Target: 5'- cGAGccGuCCGAGGCGGCCgGGUuCCa- -3' miRNA: 3'- -UUCacC-GGCUCCGCCGGgCUAuGGag -5' |
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| 22622 | 3' | -61.2 | NC_005091.1 | + | 7206 | 0.66 | 0.388358 |
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Target: 5'- ---cGGCCGGcagauGGCGGCgCGuUACCUUc -3' miRNA: 3'- uucaCCGGCU-----CCGCCGgGCuAUGGAG- -5' |
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| 22622 | 3' | -61.2 | NC_005091.1 | + | 52763 | 0.67 | 0.330413 |
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Target: 5'- --cUGGCCGGcGuGCGcGCCCGucuggucgugucGUACCUCa -3' miRNA: 3'- uucACCGGCU-C-CGC-CGGGC------------UAUGGAG- -5' |
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| 22622 | 3' | -61.2 | NC_005091.1 | + | 12589 | 0.68 | 0.307604 |
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Target: 5'- -cGUGGCCGAuucGCGcGCCCGG--UCUCg -3' miRNA: 3'- uuCACCGGCUc--CGC-CGGGCUauGGAG- -5' |
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| 22622 | 3' | -61.2 | NC_005091.1 | + | 25832 | 0.68 | 0.300273 |
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Target: 5'- aAAGUGGCCGAGaCcGCCCGuGUACUg- -3' miRNA: 3'- -UUCACCGGCUCcGcCGGGC-UAUGGag -5' |
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| 22622 | 3' | -61.2 | NC_005091.1 | + | 54994 | 0.69 | 0.25912 |
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Target: 5'- ---cGGCCG-GGCGGCCCGccgGUAUCcCg -3' miRNA: 3'- uucaCCGGCuCCGCCGGGC---UAUGGaG- -5' |
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| 22622 | 3' | -61.2 | NC_005091.1 | + | 26676 | 0.7 | 0.217057 |
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Target: 5'- cGGcGGCCGGcGGCGGCCgGG-ACUUCg -3' miRNA: 3'- uUCaCCGGCU-CCGCCGGgCUaUGGAG- -5' |
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| 22622 | 3' | -61.2 | NC_005091.1 | + | 19978 | 0.72 | 0.16283 |
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Target: 5'- cGAGUGcGCCGAGGCgGGUUCGAagGCCg- -3' miRNA: 3'- -UUCAC-CGGCUCCG-CCGGGCUa-UGGag -5' |
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| 22622 | 3' | -61.2 | NC_005091.1 | + | 37833 | 1.07 | 0.000333 |
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Target: 5'- aAAGUGGCCGAGGCGGCCCGAUACCUCg -3' miRNA: 3'- -UUCACCGGCUCCGCCGGGCUAUGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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