Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22623 | 5' | -55.9 | NC_005091.1 | + | 52640 | 0.66 | 0.700121 |
Target: 5'- -gCGUGCGUCg-CGCCGaUCccGUGuGCg -3' miRNA: 3'- aaGCACGCAGaaGCGGC-AGa-CGCuCG- -5' |
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22623 | 5' | -55.9 | NC_005091.1 | + | 55428 | 0.66 | 0.656641 |
Target: 5'- cUUCGcaauaGCGUCgcggcCGCCGUCaGcCGGGCg -3' miRNA: 3'- -AAGCa----CGCAGaa---GCGGCAGaC-GCUCG- -5' |
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22623 | 5' | -55.9 | NC_005091.1 | + | 36508 | 0.67 | 0.590935 |
Target: 5'- -cCGUGCGcauggaagCGCCGUgCUGCG-GCg -3' miRNA: 3'- aaGCACGCagaa----GCGGCA-GACGCuCG- -5' |
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22623 | 5' | -55.9 | NC_005091.1 | + | 6754 | 0.67 | 0.590935 |
Target: 5'- -aCGagGCgGUCcaCGCCGUCgaucuUGCGAGCg -3' miRNA: 3'- aaGCa-CG-CAGaaGCGGCAG-----ACGCUCG- -5' |
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22623 | 5' | -55.9 | NC_005091.1 | + | 29952 | 0.68 | 0.580052 |
Target: 5'- -gCGUGCGUaccCUUCGCgGUCgagaucaccGaCGAGCg -3' miRNA: 3'- aaGCACGCA---GAAGCGgCAGa--------C-GCUCG- -5' |
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22623 | 5' | -55.9 | NC_005091.1 | + | 50427 | 0.68 | 0.578966 |
Target: 5'- -cCGUGaaaaCUUCGCCGUUcgcacggUGCGAGUa -3' miRNA: 3'- aaGCACgca-GAAGCGGCAG-------ACGCUCG- -5' |
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22623 | 5' | -55.9 | NC_005091.1 | + | 14287 | 0.68 | 0.569212 |
Target: 5'- -aCGaUGCGUaucggCUUCGCgguguCGUCgGCGAGCg -3' miRNA: 3'- aaGC-ACGCA-----GAAGCG-----GCAGaCGCUCG- -5' |
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22623 | 5' | -55.9 | NC_005091.1 | + | 28278 | 0.69 | 0.49516 |
Target: 5'- cUCG-GCGUC-UCGCCGaCgaauaucGCGGGCg -3' miRNA: 3'- aAGCaCGCAGaAGCGGCaGa------CGCUCG- -5' |
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22623 | 5' | -55.9 | NC_005091.1 | + | 44214 | 0.69 | 0.48594 |
Target: 5'- -gCG-GCGUCUUCGCCugcguugucuucugCUGCGAGa -3' miRNA: 3'- aaGCaCGCAGAAGCGGca------------GACGCUCg -5' |
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22623 | 5' | -55.9 | NC_005091.1 | + | 53060 | 0.7 | 0.464756 |
Target: 5'- aUgGUGCGgggUUCGCUGaUCgcgGCGAGCa -3' miRNA: 3'- aAgCACGCag-AAGCGGC-AGa--CGCUCG- -5' |
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22623 | 5' | -55.9 | NC_005091.1 | + | 18388 | 0.7 | 0.454838 |
Target: 5'- cUCGUGuCGcUCgaaUUGCCGUCUuGCGAGa -3' miRNA: 3'- aAGCAC-GC-AGa--AGCGGCAGA-CGCUCg -5' |
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22623 | 5' | -55.9 | NC_005091.1 | + | 16497 | 0.7 | 0.445036 |
Target: 5'- -cCGcGCGUaCUgcUUGCCGUCUGCcGGCg -3' miRNA: 3'- aaGCaCGCA-GA--AGCGGCAGACGcUCG- -5' |
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22623 | 5' | -55.9 | NC_005091.1 | + | 49558 | 0.7 | 0.425793 |
Target: 5'- gUCGgggGCGUCUUCGuaCCGcCUGCGcGGUu -3' miRNA: 3'- aAGCa--CGCAGAAGC--GGCaGACGC-UCG- -5' |
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22623 | 5' | -55.9 | NC_005091.1 | + | 33359 | 0.71 | 0.371171 |
Target: 5'- -gCGUcGCGUCcgUCGCCGa--GCGAGCg -3' miRNA: 3'- aaGCA-CGCAGa-AGCGGCagaCGCUCG- -5' |
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22623 | 5' | -55.9 | NC_005091.1 | + | 21700 | 0.74 | 0.263315 |
Target: 5'- gUCGgGCGUUUgCGUCGUCUGCGuauAGCg -3' miRNA: 3'- aAGCaCGCAGAaGCGGCAGACGC---UCG- -5' |
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22623 | 5' | -55.9 | NC_005091.1 | + | 38119 | 1.1 | 0.000786 |
Target: 5'- cUUCGUGCGUCUUCGCCGUCUGCGAGCa -3' miRNA: 3'- -AAGCACGCAGAAGCGGCAGACGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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