Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22624 | 5' | -59.1 | NC_005091.1 | + | 23226 | 0.72 | 0.21473 |
Target: 5'- cCGUGCCGCUGUGguagCGCgGCGCGu -3' miRNA: 3'- aGCGCGGCGACGCaca-GCGaUGCGUc -5' |
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22624 | 5' | -59.1 | NC_005091.1 | + | 40957 | 0.78 | 0.09339 |
Target: 5'- cCGCGUcccgcccagCGCUGCGUG-CGCUGCGCGc -3' miRNA: 3'- aGCGCG---------GCGACGCACaGCGAUGCGUc -5' |
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22624 | 5' | -59.1 | NC_005091.1 | + | 19232 | 0.8 | 0.067557 |
Target: 5'- gCGCGCaagugaugUGCUGCGUGUCGCUgaucuucaggccguuGCGCAGc -3' miRNA: 3'- aGCGCG--------GCGACGCACAGCGA---------------UGCGUC- -5' |
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22624 | 5' | -59.1 | NC_005091.1 | + | 39416 | 1.09 | 0.000436 |
Target: 5'- aUCGCGCCGCUGCGUGUCGCUACGCAGa -3' miRNA: 3'- -AGCGCGGCGACGCACAGCGAUGCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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