miRNA: 3'- uucCUGACU-GCaCUUCAAUGAGGGCU- -5'
miRNA: 3'- -UUCCUGacuGCaCUUCAAUGAGGGCU- -5'
miRNA: 3'- uUCCUGACUgcaCUUCAAUGAGGGCu -5'
miRNA: 3'- uuCCUGaCU-GCaCUUCAAUGAGGGCU- -5'
miRNA: 3'- -UUCCUGACUGCACUUCAAUGAGGGCU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22625 | 3' | -51.4 | NC_005091.1 | + | 8783 | 0.67 | 0.835362 |
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Target: 5'- --uGGCUGAcCG-GAAGUUAUUUCCGAc -3' miRNA: 3'- uucCUGACU-GCaCUUCAAUGAGGGCU- -5' |
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| 22625 | 3' | -51.4 | NC_005091.1 | + | 39821 | 0.68 | 0.806923 |
|
Target: 5'- gGAGGAU---CG-GAAGUUACUUCCGAu -3' miRNA: 3'- -UUCCUGacuGCaCUUCAAUGAGGGCU- -5' |
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| 22625 | 3' | -51.4 | NC_005091.1 | + | 6337 | 0.7 | 0.690169 |
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Target: 5'- cAGGACUGAaacGAAGUUACUUCUGc -3' miRNA: 3'- uUCCUGACUgcaCUUCAAUGAGGGCu -5' |
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| 22625 | 3' | -51.4 | NC_005091.1 | + | 54897 | 0.7 | 0.690169 |
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Target: 5'- -cGGACgGGuCG-GAAGUUACUUCCGGu -3' miRNA: 3'- uuCCUGaCU-GCaCUUCAAUGAGGGCU- -5' |
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| 22625 | 3' | -51.4 | NC_005091.1 | + | 39938 | 1.07 | 0.003452 |
|
Target: 5'- cAAGGACUGACGUGAAGUUACUCCCGAu -3' miRNA: 3'- -UUCCUGACUGCACUUCAAUGAGGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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