Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22634 | 3' | -58.5 | NC_005091.1 | + | 31875 | 0.66 | 0.555266 |
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Target: 5'- -aCCGUGUcgucgCGCUCGUcgGCGgccugcucGUGCCGUg -3' miRNA: 3'- gaGGCACA-----GCGAGCA--CGU--------CACGGCGu -5' |
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| 22634 | 3' | -58.5 | NC_005091.1 | + | 31415 | 0.66 | 0.534295 |
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Target: 5'- -cCCGUucGUCGCgccgaaCGUGCAagGCCGCGu -3' miRNA: 3'- gaGGCA--CAGCGa-----GCACGUcaCGGCGU- -5' |
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| 22634 | 3' | -58.5 | NC_005091.1 | + | 54647 | 0.66 | 0.523914 |
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Target: 5'- gUCCGUGUCGgUCGgucuuCAGUacGaCCGCAc -3' miRNA: 3'- gAGGCACAGCgAGCac---GUCA--C-GGCGU- -5' |
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| 22634 | 3' | -58.5 | NC_005091.1 | + | 55586 | 0.68 | 0.425249 |
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Target: 5'- gUCCacg-CGCUCGUGCAG-GCgGCGa -3' miRNA: 3'- gAGGcacaGCGAGCACGUCaCGgCGU- -5' |
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| 22634 | 3' | -58.5 | NC_005091.1 | + | 19666 | 0.7 | 0.338519 |
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Target: 5'- -gUCGUGUgGCUCGUGCucaugGUCGCGu -3' miRNA: 3'- gaGGCACAgCGAGCACGuca--CGGCGU- -5' |
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| 22634 | 3' | -58.5 | NC_005091.1 | + | 30261 | 0.72 | 0.227962 |
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Target: 5'- -cCCGUGgCGCUCGUGCAGaUGCuCGa- -3' miRNA: 3'- gaGGCACaGCGAGCACGUC-ACG-GCgu -5' |
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| 22634 | 3' | -58.5 | NC_005091.1 | + | 48796 | 1.08 | 0.000622 |
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Target: 5'- uCUCCGUGUCGCUCGUGCAGUGCCGCAu -3' miRNA: 3'- -GAGGCACAGCGAGCACGUCACGGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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