Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22636 | 5' | -58.5 | NC_005091.1 | + | 51997 | 0.66 | 0.525538 |
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Target: 5'- uUGCUGUUuUCCUucGUGCCGCGCc- -3' miRNA: 3'- uAUGGCAAcGGGAcuCACGGCGCGag -5' |
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| 22636 | 5' | -58.5 | NC_005091.1 | + | 12452 | 0.66 | 0.515069 |
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Target: 5'- -cGCCGUcGCaCUG---GCCGCGCUCg -3' miRNA: 3'- uaUGGCAaCGgGACucaCGGCGCGAG- -5' |
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| 22636 | 5' | -58.5 | NC_005091.1 | + | 34334 | 0.67 | 0.454245 |
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Target: 5'- --uCCaGUaGCCCUGcGgccgGCCGCGCUUg -3' miRNA: 3'- uauGG-CAaCGGGACuCa---CGGCGCGAG- -5' |
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| 22636 | 5' | -58.5 | NC_005091.1 | + | 11956 | 0.68 | 0.415928 |
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Target: 5'- -aGCCGUcGUCCUGcgugacGGUGaCGUGCUCg -3' miRNA: 3'- uaUGGCAaCGGGAC------UCACgGCGCGAG- -5' |
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| 22636 | 5' | -58.5 | NC_005091.1 | + | 51689 | 0.68 | 0.397527 |
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Target: 5'- cAUGCCGaaucgcGCCC---GUGCCGCGCUUc -3' miRNA: 3'- -UAUGGCaa----CGGGacuCACGGCGCGAG- -5' |
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| 22636 | 5' | -58.5 | NC_005091.1 | + | 49489 | 0.69 | 0.345565 |
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Target: 5'- -gAUCGaaaUUGCUaaGAGUGCCGCGCg- -3' miRNA: 3'- uaUGGC---AACGGgaCUCACGGCGCGag -5' |
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| 22636 | 5' | -58.5 | NC_005091.1 | + | 18969 | 0.69 | 0.329361 |
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Target: 5'- ---gCGUUGCCUUGAaUGCCGCcCUCc -3' miRNA: 3'- uaugGCAACGGGACUcACGGCGcGAG- -5' |
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| 22636 | 5' | -58.5 | NC_005091.1 | + | 46304 | 0.7 | 0.276422 |
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Target: 5'- -gGCCGUcuccgaccacucgUGCCa-GAGUucgagGCCGCGCUCg -3' miRNA: 3'- uaUGGCA-------------ACGGgaCUCA-----CGGCGCGAG- -5' |
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| 22636 | 5' | -58.5 | NC_005091.1 | + | 49929 | 1.08 | 0.000485 |
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Target: 5'- gAUACCGUUGCCCUGAGUGCCGCGCUCa -3' miRNA: 3'- -UAUGGCAACGGGACUCACGGCGCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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