Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2264 | 5' | -54.8 | NC_001405.1 | + | 23974 | 0.66 | 0.595491 |
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Target: 5'- -cCGCGCGcccccaaaaagcgGCUGaGGCGGCGUcUCGa- -3' miRNA: 3'- uaGCGCGU-------------UGAC-CCGUCGUAaAGCgc -5' |
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| 2264 | 5' | -54.8 | NC_001405.1 | + | 15770 | 0.66 | 0.562272 |
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Target: 5'- uGUUGCGCAGC--GGUAGCGUccCGUGa -3' miRNA: 3'- -UAGCGCGUUGacCCGUCGUAaaGCGC- -5' |
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| 2264 | 5' | -54.8 | NC_001405.1 | + | 12656 | 0.66 | 0.550927 |
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Target: 5'- -gUGCGCGagGCcgUGGcGCAGCGUgagCGCGc -3' miRNA: 3'- uaGCGCGU--UG--ACC-CGUCGUAaa-GCGC- -5' |
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| 2264 | 5' | -54.8 | NC_001405.1 | + | 13294 | 0.67 | 0.527343 |
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Target: 5'- -aUGCGCGACgGGGUaacgcccAGCGUggCGCu -3' miRNA: 3'- uaGCGCGUUGaCCCG-------UCGUAaaGCGc -5' |
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| 2264 | 5' | -54.8 | NC_001405.1 | + | 5214 | 0.69 | 0.383642 |
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Target: 5'- uUCGCGgGuugGGGCGGC-UUUCGCu -3' miRNA: 3'- uAGCGCgUugaCCCGUCGuAAAGCGc -5' |
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| 2264 | 5' | -54.8 | NC_001405.1 | + | 8519 | 0.72 | 0.267669 |
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Target: 5'- -cCGCGCGGC-GGGCGGUGggcCGCGg -3' miRNA: 3'- uaGCGCGUUGaCCCGUCGUaaaGCGC- -5' |
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| 2264 | 5' | -54.8 | NC_001405.1 | + | 27502 | 0.72 | 0.260569 |
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Target: 5'- --gGCGCAGCUugcGGGCGGC-UUUCGUc -3' miRNA: 3'- uagCGCGUUGA---CCCGUCGuAAAGCGc -5' |
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| 2264 | 5' | -54.8 | NC_001405.1 | + | 15273 | 0.75 | 0.176612 |
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Target: 5'- gGUUGCGCAGCggGGGCGGCAgcuucugcCGCu -3' miRNA: 3'- -UAGCGCGUUGa-CCCGUCGUaaa-----GCGc -5' |
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| 2264 | 5' | -54.8 | NC_001405.1 | + | 20868 | 1.08 | 0.000633 |
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Target: 5'- cAUCGCGCAACUGGGCAGCAUUUCGCGg -3' miRNA: 3'- -UAGCGCGUUGACCCGUCGUAAAGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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