Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22640 | 3' | -56 | NC_005091.1 | + | 51520 | 0.66 | 0.644 |
Target: 5'- cAUCGCgaGCCGuagaaccGCCCccGCGCAg -3' miRNA: 3'- -UAGCGaaCGGCuaaa---CGGGcaCGCGU- -5' |
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22640 | 3' | -56 | NC_005091.1 | + | 51274 | 0.66 | 0.610683 |
Target: 5'- aAUCGUc-GCCGucgcGCCCGUGgGCGa -3' miRNA: 3'- -UAGCGaaCGGCuaaaCGGGCACgCGU- -5' |
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22640 | 3' | -56 | NC_005091.1 | + | 51707 | 0.67 | 0.560009 |
Target: 5'- -cCGCUucauucggcauaucaUGCCGAaucgcGCCCGUGcCGCGc -3' miRNA: 3'- uaGCGA---------------ACGGCUaaa--CGGGCAC-GCGU- -5' |
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22640 | 3' | -56 | NC_005091.1 | + | 55225 | 0.68 | 0.534013 |
Target: 5'- gGUCgGCUcGuUCGAcgcgGCCCGUGCGCGg -3' miRNA: 3'- -UAG-CGAaC-GGCUaaa-CGGGCACGCGU- -5' |
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22640 | 3' | -56 | NC_005091.1 | + | 20270 | 0.68 | 0.523306 |
Target: 5'- -aCGCgucGCCGcUUUGCgaguagccgCCGUGCGCGa -3' miRNA: 3'- uaGCGaa-CGGCuAAACG---------GGCACGCGU- -5' |
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22640 | 3' | -56 | NC_005091.1 | + | 44443 | 0.68 | 0.491711 |
Target: 5'- -aCGCUUGCCGGUgcacccGCCaGUGcCGCu -3' miRNA: 3'- uaGCGAACGGCUAaa----CGGgCAC-GCGu -5' |
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22640 | 3' | -56 | NC_005091.1 | + | 24024 | 0.69 | 0.481375 |
Target: 5'- uUCGCgu-CCGGgaUGCCCGUGCcucGCAc -3' miRNA: 3'- uAGCGaacGGCUaaACGGGCACG---CGU- -5' |
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22640 | 3' | -56 | NC_005091.1 | + | 34261 | 0.69 | 0.471145 |
Target: 5'- aGUCGCgcggcaUUGCCGAUaucgUGUCCGgccugaaggacaUGCGCAu -3' miRNA: 3'- -UAGCG------AACGGCUAa---ACGGGC------------ACGCGU- -5' |
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22640 | 3' | -56 | NC_005091.1 | + | 52635 | 0.69 | 0.451024 |
Target: 5'- cGUCGC--GCCGAU---CCCGUGUGCGc -3' miRNA: 3'- -UAGCGaaCGGCUAaacGGGCACGCGU- -5' |
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22640 | 3' | -56 | NC_005091.1 | + | 36523 | 0.71 | 0.358135 |
Target: 5'- uAUCGUgcGCCGAUa---CCGUGCGCAu -3' miRNA: 3'- -UAGCGaaCGGCUAaacgGGCACGCGU- -5' |
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22640 | 3' | -56 | NC_005091.1 | + | 48790 | 0.71 | 0.332189 |
Target: 5'- uGUCGCUcgugcagUGCCGcAUUggccGgCCGUGCGCAc -3' miRNA: 3'- -UAGCGA-------ACGGC-UAAa---CgGGCACGCGU- -5' |
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22640 | 3' | -56 | NC_005091.1 | + | 51047 | 1.06 | 0.001121 |
Target: 5'- gAUCGCUUGCCGAUUUGCCCGUGCGCAc -3' miRNA: 3'- -UAGCGAACGGCUAAACGGGCACGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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