Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22640 | 5' | -58.7 | NC_005091.1 | + | 13137 | 0.66 | 0.512552 |
Target: 5'- -aUGCUGACGgGGCcguAUGGCuGGUc -3' miRNA: 3'- caGCGACUGCgCCGuu-UGCCGcCCAu -5' |
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22640 | 5' | -58.7 | NC_005091.1 | + | 55219 | 0.66 | 0.492137 |
Target: 5'- cUCGUUcGACGCGGCccguGCGcGgGGGUc -3' miRNA: 3'- cAGCGA-CUGCGCCGuu--UGC-CgCCCAu -5' |
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22640 | 5' | -58.7 | NC_005091.1 | + | 46021 | 0.67 | 0.48207 |
Target: 5'- -cCGCgccgGaACGCaGCGAgaGCGGCGGGg- -3' miRNA: 3'- caGCGa---C-UGCGcCGUU--UGCCGCCCau -5' |
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22640 | 5' | -58.7 | NC_005091.1 | + | 17920 | 0.67 | 0.462244 |
Target: 5'- cGUCGCcucgGucgUGCGGUAGACGGCcggcgcgacGGGUGu -3' miRNA: 3'- -CAGCGa---Cu--GCGCCGUUUGCCG---------CCCAU- -5' |
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22640 | 5' | -58.7 | NC_005091.1 | + | 53085 | 0.67 | 0.462244 |
Target: 5'- gGUCGCgagcauUGCGGCGAcCGuuuGCGGGUAg -3' miRNA: 3'- -CAGCGacu---GCGCCGUUuGC---CGCCCAU- -5' |
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22640 | 5' | -58.7 | NC_005091.1 | + | 4823 | 0.67 | 0.442856 |
Target: 5'- -gCGCUcGGCGCGGCGgcAACGgucaaGCGGGc- -3' miRNA: 3'- caGCGA-CUGCGCCGU--UUGC-----CGCCCau -5' |
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22640 | 5' | -58.7 | NC_005091.1 | + | 4974 | 0.67 | 0.442856 |
Target: 5'- aGUUGCUGGaaCGGCGAuaACGGUgcucGGGUAu -3' miRNA: 3'- -CAGCGACUgcGCCGUU--UGCCG----CCCAU- -5' |
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22640 | 5' | -58.7 | NC_005091.1 | + | 26686 | 0.69 | 0.3702 |
Target: 5'- cUUGCUGcUGCGGCGGcCGGCGGcGg- -3' miRNA: 3'- cAGCGACuGCGCCGUUuGCCGCC-Cau -5' |
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22640 | 5' | -58.7 | NC_005091.1 | + | 40713 | 0.69 | 0.3702 |
Target: 5'- uUCGC-GACGCGGCuguACGGCGucgaGUAc -3' miRNA: 3'- cAGCGaCUGCGCCGuu-UGCCGCc---CAU- -5' |
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22640 | 5' | -58.7 | NC_005091.1 | + | 53535 | 0.7 | 0.329113 |
Target: 5'- -cCGCacgGGCGCGGCuuucUGGCGGGa- -3' miRNA: 3'- caGCGa--CUGCGCCGuuu-GCCGCCCau -5' |
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22640 | 5' | -58.7 | NC_005091.1 | + | 53049 | 0.72 | 0.2446 |
Target: 5'- uUCGCUGAuCGCGGCGAGCauGGCGa--- -3' miRNA: 3'- cAGCGACU-GCGCCGUUUG--CCGCccau -5' |
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22640 | 5' | -58.7 | NC_005091.1 | + | 384 | 0.74 | 0.178914 |
Target: 5'- cGUCGUgaAgGCGGcCAAGCGGCGGGg- -3' miRNA: 3'- -CAGCGacUgCGCC-GUUUGCCGCCCau -5' |
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22640 | 5' | -58.7 | NC_005091.1 | + | 57778 | 0.74 | 0.178914 |
Target: 5'- cGUCGUgaAgGCGGcCAAGCGGCGGGg- -3' miRNA: 3'- -CAGCGacUgCGCC-GUUUGCCGCCCau -5' |
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22640 | 5' | -58.7 | NC_005091.1 | + | 51010 | 1.06 | 0.000762 |
Target: 5'- gGUCGCUGACGCGGCAAACGGCGGGUAu -3' miRNA: 3'- -CAGCGACUGCGCCGUUUGCCGCCCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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